2种方法提取香蜂花叶挥发性成分的GC-TOFMS分析

目的]分析研究香蜂花叶挥发性成分含量,探讨不同提取方法提取香蜂花叶中的挥发性成分的差异。方法]采用GC-TOFMS仪分析测定,由同时蒸馏萃取法和超声波辅助提取法分别提取的香蜂花叶挥发油,用峰面积归一法计算各个组分相对含量。结果]从同时蒸馏萃取物中分离出93种成分,鉴定出41种成分,占化合物检出总量的89.79%;其中含有的最主要成分为香叶醛(24.54%);从超声波辅助提取物中分离出67种成分,鉴定出31种成分,占化合物检出总量的92.26%;其中含有的最主要成分为棕榈酸(30.80%)。结论]不同提取方法提取的挥发性成分的组成和相对含量存在明显不同。

GC-TOFMS Analysis of the Volatile Components from Leaves of Melissa officinalis L. by Two Extraction Methods

Objective] To study on the volatile component in Melissa officinalis L.leaves and to compare the volatile components extracted from Melissa officinalis L.leaves by two extraction methods.Method] The volatile components from Melissa officinalis L.extracted by using simultaneous distillation extraction and ultrasound-assisted extraction were identified by GC-TOFMS and computer spectrum search.The effects of different extraction methods on volatile components were also compared.Result] 93 components were separated from Melissa officinalis L.leaves by using simultaneous distillation extraction and 41 of them were identified,accounting for about 89.79% of the total content.The most abundant constituent was gerznial(24.54%).67 components were separated by using simultaneous distillation extraction and 31 of them were identified,accounting for about 92.26% of the total content.The most abundant constituent was palmitic acid(30.80%).Conclusion] There were significant differences in the volatile components and its relative contents in Melissa officinalis L.leaves by different extraction methods.