| 不同培养条件对草麻黄愈伤组织麻黄碱含量的影响 |
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| 引用本文： | 张红梅,王明艳,蔡宝宏,信佳言. 不同培养条件对草麻黄愈伤组织麻黄碱含量的影响[J]. 安徽农业科学, 2012, 0(5): 2640+2653 |
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| 作者姓名： | 张红梅 王明艳 蔡宝宏 信佳言 |
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| 作者单位： | 河北联合大学生命科学学院,河北唐山,063000;唐山市第一中学生物教研室,河北唐山,063000;河北联合大学基础医学院,河北唐山,063000 |
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| 基金项目： | 唐山市"草麻黄体外培养高产药用成分的研究"课题 |
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| 摘 要： | [目的]探寻适宜草麻黄愈伤组织麻黄碱生成的最佳培养条件。[方法]采用乙醚抽提法提取麻黄碱,用紫外分光光度计测定麻黄碱含量,分别试验水解酪蛋白浓度、基本培养基、取材时间、摇床转速对麻黄碱含量的影响。[结果]适宜草麻黄愈伤组织麻黄碱生成的条件为以MS为基本培养基,以继代培养25 d的愈伤组织为材料,添加300.0 mg/L的水解酪蛋白,在转速110 r/min的摇床上悬浮培养。[结论]该研究为麻黄碱的植物细胞体外工厂化生产奠定了基础。
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| 关 键 词： | 草麻黄 悬浮培养 麻黄碱 愈伤组织 |
Effects of Different Culture Conditions on Callus Ephedrine Production in Ephedrae sinica Stapf |
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| Affiliation: | ZHANG Hong-mei et al(College of Life Science,Hebei United University,Tangshan,Hebei 063000) |
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| Abstract: | [Objective]To explore the optimal culture conditions for producing ephedrine from Ephedrae sinica Stapf callus.[Method]Ephedrine was extracted with petroleum ether,its content was determined by using ultraviolet spectrophotometer,the effects of casein hydrolysate concentration,basic medium,sampling time and shaker speed on ephedrine content were experimentally studied.[Result]The best conditions for producing ephedrine from E.sinica Stapf callus were MS basal culture medium,300.0 mg/L casein hydrolysate,callus generation of 25 days,culture rotating speed of 110 r/min.[Conclusion]The study laid foundation for the industrialized production of ephedrine. |
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| Keywords: | Ephedrae sinica Stapf Suspension culture Ephedrine Callus |
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