不同溶剂索氏提取的白花丹叶中白花丹醌的含量测定 |
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引用本文: | 杜泽乡,谭宁昌,罗朝晖.不同溶剂索氏提取的白花丹叶中白花丹醌的含量测定[J].安徽农业科学,2012(5):2628-2629,2639. |
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作者姓名: | 杜泽乡 谭宁昌 罗朝晖 |
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作者单位: | 桂林医学院,广西桂林,541004 |
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摘 要: | 目的]测定不同方法提取的白花丹鲜叶中白花丹醌的含量并加以比较,优选出最佳提取溶剂。方法]采用索氏提取法,选用不同的溶剂提取白花丹鲜叶中的白花丹醌,并通过HPLC测定其含量,从而确定最佳提取溶剂。结果]测得用氯仿、石油醚(II)、浓度95%乙醇、无水甲醇提取白花丹醌的含量依次为0.511%、0.341%、0.233%和0.191%;用氯仿提取的白花丹醌含量(提取率)最高。结论]不同方法提取的白花丹鲜叶中白花丹醌的含量差异明显。
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关 键 词: | 白花丹鲜叶 白花丹醌 索氏提取 HPLC |
Content Determination of Plumbagin in the Leaves of Plumbago zeylanica L. by Soxhlet Extraction with Different Solvents |
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Institution: | DU Ze-xiang et al(Guilin Medical College,Guilin,Guangxi 541004) |
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Abstract: | Objective] To determine and compare the contents of plumbagin in the leaves of Plumbago zeylanica L.by different extraction methods,and to select the optimal extraction solvent.Method] Soxhlet extraction method was adopted;plumbagin in the leaves of P.zeylanica was extracted by different solvents.Its content was detected by HPLC,so as to select the optimal extraction solvent.Result] Contents of plumbagin extracted by chloroform,petroleum ether(II),95% ethanol and anhydrous methanol were 0.511%,0.341%,0.233% and 0.191%,respectively.Content of plumbagin in P.zeylanica extracted by chloroform was the highest.Conclusion] There were significant differences in the content of plumbagin in P.zeylanica fresh leaves by different extraction methods. |
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Keywords: | Fresh leaves of Plumbago zeylanica L Plumbagin Soxhlet extraction HPLC |
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