省沽油组培过程中外植体灭菌技术的研究

目的]寻求省沽油组织培养中适宜的外植体灭菌方法。方法]研究了70%乙醇、0.1%HgCl2、0.1%HgCl2+70%乙醇组合在不同灭菌时间内对外植体生长的影响。结果]适宜的外植体灭菌技术:茎尖用1%HgCl2+70%乙醇处理时间分别为5 min+50 s,成活率100%;茎段用1%HgCl2处理时间为15 m,成活率95%;幼叶用1%HgCl2处理时间为12 min,成活率85%。结论]1%HgCl2+70%乙醇、1%HgCl2是省沽油外植体的有效灭菌剂。

Research on Explants Sterilization Technique in Staphylea bumalda DC.Tissue Culture Process

Objective] The aim was to seek appropriate explants sterilization method in Staphylea bumalda DC.tissue culture process.Method] The comparative method was used to study the effect of alcohol,HgCl2,HgCl2+alcohol combination in different sterilization time on explants growth.Result] The results showed that the appropriate explant sterilization technology was the shoot tips survival rate was 100% with 1% HgCl2+70% alcohol,processing time was 5 min+50 s respectively;The stem sections survival rate was 95% with 1% HgCl2,processing time was 15 min;The young leaves survival rate was 85% with 1% HgCl2,processing time was 12 min.Conclusion] 1% HgCl2+70% alcohol,1% HgCl2 was effective sterilant of Staphylea bumalda DC.explants.