怀地黄试管苗的快繁体系研究

[目的]建立怀地黄试管苗的快繁体系。[方法]分别进行了NAA、6-BA和KT对茎尖培养以及NAA、6-BA试管苗快速繁殖的试验。[结果]中低浓度的NAA和6-BA可以在一定程度上促进怀地黄试管苗的快速繁殖,而高浓度的NAA和6-BA则会抑制怀地黄试管苗的快速繁殖;优选出的怀地黄试管苗的快速繁殖的最佳培养基为MS+0.002 mg/L NAA+0.2 mg/L 6-BA。[结论]试验优选出了怀地黄试管苗的快速繁殖的最佳培养基,为怀地黄的开发利用提供了理论依据。

Study on Rapid Propagation System of Rehmannia glutinosa f. hueichingensis (Chan et Sehih) Hsiao Test-Tube Plantlet

[Objective] To establish the rapid propagation system of Rehmannia glutinosa f.hueichingensis(Chan et Sehih) Hsiao test-tube plantlet.[Method] In rapid propagation test,the stem tip of Rehmannia glutinosa f.hueichingensis(Chan et Sehih) Hsiao test-tube plantlet was respectively cultured in medium containing NAA,6-BA and KT at different concentrations.[Result] At low and medium concentration,NAA and 6-BA could promote the rapid propagation of Rehmannia glutinosa f.hueichingensis(Chan et Sehih) Hsiao test-tube plantlet to some extent.On the contrary,high concentration of NAA and 6-BA would inhibit the rapid propagation of test-tube plantlet.The optimal medium for test-tube plantlet was MS+0.002 mg/L NAA+0.2 mg/L 6-BA.[Conclusion] This study could provide theoretical basis for the development and utilization of Rehmannia glutinosa f.hueichingensis(Chan et Sehih) Hsiao.