| 黑曲霉D226乳糖酶分离纯化研究 |
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| 引用本文： | 李红飞,于宏伟,韩军,李宁,林杨,贾英民. 黑曲霉D226乳糖酶分离纯化研究[J]. 河北农业大学学报, 2006, 29(5): 65-68 |
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| 作者姓名： | 李红飞 于宏伟 韩军 李宁 林杨 贾英民 |
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| 作者单位： | 河北农业大学,食品科技学院,河北,保定,071001;河北农业大学,食品科技学院,河北,保定,071001;河北农业大学,食品科技学院,河北,保定,071001;河北农业大学,食品科技学院,河北,保定,071001;河北农业大学,食品科技学院,河北,保定,071001;河北农业大学,食品科技学院,河北,保定,071001 |
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| 基金项目： | 河北省农副产品加工重大科技专项项目 |
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| 摘 要： | 为从黑曲霉D2-26发酵液中得到单一的乳糖酶组分,以便研究其酶学性质。试验通过采用过滤、超滤、硫酸铵分级盐析、Sephadex G-25脱盐、DEAE-纤维素-32离子强度梯度层析、Sephadex G-100凝胶过滤、DEAE-纤维素-32 pH梯度层析等方法从发酵液中分离纯化乳糖酶,纯化酶液经PAGE电泳鉴定得到单一蛋白条带。为此建立了一套简单易行的黑曲霉D2-26乳糖酶分离纯化方法。
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| 关 键 词： | 乳糖酶 离子交换层析 分子筛层析 分离纯化 |
| 文章编号： | 1000-1573（2006）05-0065-04 |
| 收稿时间： | 2006-03-03 |
| 修稿时间： | 2006-03-03 |
Study of isolation and purification of lactase from Aspergillus niger D2-26 |
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| LI Hong-fei,YU Hong-wei,HAN Jun,Li Ning,LIN Yang,JIA Ying-min. Study of isolation and purification of lactase from Aspergillus niger D2-26[J]. Journal of Agricultural University of Hebei, 2006, 29(5): 65-68 |
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| Authors: | LI Hong-fei YU Hong-wei HAN Jun Li Ning LIN Yang JIA Ying-min |
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| Affiliation: | College of Food Science and Technology, Agricultural University of Hebei, Baoding 071001, China |
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| Abstract: | In order to get a single lactase component from the Aspergillus niger D2-26 fermentation liquor to study the enzyme properties,this study was done by means of filtration,ultra-filtration,ammonium sulfate fraction,sephadex G-25 gel filtration,DEAE ion-exchange chromatography,sephadex G100 gel filtration to isolate and purify the lactase from the fermentation liquor.The purified enzyme identified by PAGE indicated that a single protein band was obtained.The research established a set of simple and feasible ways in the isolation and purification of lactase from Aspergillus niger D2-26 fermentation liquor. |
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| Keywords: | lactase ion-change chromatography gel filtration chromatography isolation and purification |
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