| 紫玉兰花干燥方法对其活性成分和抗氧化性的影响 |
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| 引用本文： | 李凤英,刘素稳,郭朔.紫玉兰花干燥方法对其活性成分和抗氧化性的影响[J].园艺学报,2015,42(6):1150-1152. |
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| 作者姓名： | 李凤英 刘素稳 郭朔 |
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| 作者单位： | 河北科技师范学院食品科技学院，河北昌黎 066600 |
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| 基金项目： | 河北省高等学校科学技术研究重点项目（ZD2014097） |
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| 摘 要： | 以紫玉兰(Magnolia liliflora Desr)花为材料,采用晒干、阴干、热风干燥、微波干燥等不同的干燥方法进行处理,探索其对紫玉兰花黄酮、多酚含量及抗氧化性的影响。结果表明:不同干燥方法处理紫玉兰花,干燥速度快慢顺序为:微波干燥热风干燥晒干阴干。阴干、900 W微波干燥和50℃热风干燥样品的黄酮、多酚含量及抗氧化性较高,晒干处理对多酚、黄酮的分解损失最大,抗氧化活性最低。DPPH·清除率与黄酮含量(R2=0.7300)和多酚含量(R2=0.6675)显著相关,总还原力与多酚含量显著相关(R2=0.8234)。
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| 关 键 词： | 紫玉兰花 干燥方法 黄酮 多酚 抗氧化性 |
Effects of Drying Methods on Chemical Composition and Antioxidant Activity of the Flowers of Magnolia liliflora |
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| LI Feng-ying,LIU Su-wen,GUO Shuo.Effects of Drying Methods on Chemical Composition and Antioxidant Activity of the Flowers of Magnolia liliflora[J].Acta Horticulturae Sinica,2015,42(6):1150-1152. |
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| Authors: | LI Feng-ying LIU Su-wen GUO Shuo |
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| Institution: | 1. Department of Physiology and Pathophysiology, School of Basic Medical Sciences, Fudan University, Shanghai 200032;
2. Department of Pharmacology, School of Pharmacy, Fudan University, Shanghai 201203, China;
3. Research Center, Montreal Heart Institute, Montreal, Canada |
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| Abstract: | AIM: To establish a perforated whole-cell patch-clamp technique with β-escin to record L-type calcium current (ICa,L) in osteoblasts.METHODS: ROS 17/2.8 is a rat osteoblast-like osteosarcoma cell line. β-escin was applied to the pipette solution to permeabilize the cell membrane and the perforated patch recording mode was obtained. RESULTS: β-escin at concentration of 50 μmol/L easily permeabilized the cell membrane and obtained a perforated patch recording mode in 2~7 min. This technique prevented ICa,L rundown and preserved cytoplasmic signaling pathways. CONCLUSION: β-escin may be used as an alternative ionophore for perforated patch-clamp studies in osteoblasts and results in minimal rundown that could facilitate recordings of ICa,L in osteoblasts. |
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| Keywords: | Magnolia liliflora drying method flavonoids polyphenols antioxidant activity |
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