Molecular characterisation of Victorian Newcastle disease virus isolates from 1976 to 1999 |
| |
| Authors: | Peroulis-Kourtis I O'Riley K Grix D Condron R J Ainsworth C |
| |
| Institution: | Department of Natural Resources and Environment, Victorian Institute of Animal Science, Attwood. irene.peroulis@nre.vic.gov.au |
| |
| Abstract: | OBJECTIVE: To characterise Newcastle disease virus isolates obtained in Victoria from 1976 to 1999 and identify the diversity of FO cleavage signal. DESIGN: RT-PCR using viral RNA extracted from positive NDV allantoic fluid was performed to amplify a segment of the NDV F and HN genes. Molecular characterisation of the nucleotide and amino acid sequences within the FO cleavage site was undertaken. RESULTS: All isolates contained 'avirulent FO cleavage signal sequence of varied amino acid composition. CONCLUSIONS: Molecular characterisation of past and present NDV FO cleavage signal sequences will provide valuable epidemiological information and assist in understanding the genetic origins and relationships of outbreaks. |
| |
| Keywords: | |
| 本文献已被 PubMed 等数据库收录！ |
