杂交棒眼果蝇亮红眼的遗传特性及其眼睛色素含量分析

黑腹果蝇眼色性状由多个基因所调控,是研究基因间互作的有效模型。为了探究棒眼果蝇与野生型果蝇杂交产生亮红眼突变体的机制,将亮红眼突变体分别与野生型果蝇进行正、反、测交和与眼色突变体果蝇(cn,v和st)进行互补测验,同时对不同果蝇品系进行了眼色素的测定。结果表明,亮红眼果蝇是由于其scarlet(猩红眼)基因突变所致,暂命名为stbr;stbr果蝇眼黄素含量极显著低于野生型果蝇(P<0.001),而其果蝇喋呤含量比较无显著差异(P>0.05)。本研究中stbr果蝇眼睛呈现亮红色,可能是由于其scarlet基因突变,影响了眼黄素前体物的跨膜运输,造成果绳眼睛中眼黄素合成水平降低所致,此结果为stbr果蝇开展分子生物学研究奠定了基础。

Genetic characteristic and eye pigment analysis of bright-red-eye outcross progeny of Bar fly

Eye colour of Drosophila melanogaster is modulated by multiple genes,which is effective model for interaction research among genes.For understand the mechanism of mutation of the bright-red-eye progeny of Bar and wildtype fly,bright-red-eye mutants crossed to wildtype flies and eye colour mutants(cn,v and st) respectively.Eye pigment analysis of different fly lines was also performed.Results of crossing indicated that mutation of scarlet gene is responsible for the bright-red-eye phenotype.The bright-red-eye mutant was named st~(br) in this research.Results of eye pigment analysis represented that st~(br) fly have siginificantly less xanthommatin than wild flies(P<0.001) but no difference of drodopterin content is found between wild type and st~(br)(P>0.05).Bright red eye colour of st~(br) fly in this research may be result of mutation scarlet gene,which induced interruptive trans-membrane transportation of pigment precursor and decreasing biosynthesis of xanthommatin.Result of this research provides basis for further molecular biological analysis of st~(br) fly.