| SCoT分子标记的研究现状及在茶树育种中的应用前景 |
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| 引用本文： | 李佼,李秀峰,陈曦,张羽. SCoT分子标记的研究现状及在茶树育种中的应用前景[J]. 茶叶通讯, 2015, 0(3): 8-12. DOI: 10.3969/j.issn.1009-525X.2015.03.002 |
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| 作者姓名： | 李佼 李秀峰 陈曦 张羽 |
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| 作者单位： | 1. 陕西省汉中市农业科学研究所,陕西 汉中,723000;2. 陕西理工学院 生物科学与工程学院,陕西 汉中,723000 |
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| 基金项目： | 陕西省科技统筹创新工程计划项目，陕西省自然科学基础研究计划项目 |
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| 摘 要： | 目标起始密码子多态性(Start codon targeted polymorphism,SCo T)是一种针对ATG起始密码子及侧翼序列保守性开发的新型目的基因分子标记,具有操作简便、多态性高、遗传稳定性好和成本低等优势,已经被广泛应用于多种植物的遗传研究。本文介绍了SCo T标记的原理、引物设计、PCR体系优化,综述了SCo T标记在多种植物中的应用进展,并展望了其在茶树育种上的应用前景。
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| 关 键 词： | SCoT 分子标记 茶树 茶树育种 |
Research Advance of SCoT Marker and Prospect of Its Application in Tea Breeding |
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| Abstract: | Start codon targeted polymorphism (SCoT) is a novel molecular marker to explore targeted gene diversity, which was designed on start codon ATG and conserved lfanking sequences. Taken the advantages of feasible operation, more informative, stable heritage and low-cost, SCoT has been widely applied in a variety of plants. Hereby, we described the principles of SCoT, primer design and optimization of SCoT-PCR system. In addition, we summarized the application advance of SCoT molecular marker in plants and discussed the future prospects of its application in tea breeding. |
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| Keywords: | SCoT molecular marker camellia sinensis tea breeding |
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