拟南芥基因组 NBS-LRR类基因家族的生物信息学分析

【研究目的】对拟南芥（Arabidopsis thaliana）中含有核苷酸结合位点（Nucleotide binding site，NBS）和富含亮氨酸的重复序列（Leucine—rich repeat，ERR）类基因进行了分析。【方法】利用TAIR、Pfam网站，数据库和Chromosome Map Tool、MEGA3．0、ClustalX软件，分析确定NBS-LRR基因及其类型、染色体物理分布、系统发育学关系。【结果】在拟南芥全基因组中共有204个NBS—LRR类基因，大多位于1号和5号染色体上，其中71．6％的NBS—LRR类基因分布在基因簇内。对NBS—LRR类基因家族进行了氨基酸多序列比对和系统进化树分析，NBS-LRR类基因家族可分为四个亚家族。另外，NBS—LRR基因在进化过程中存在较多的复制现象。

Bioinformatic Analysis of the NBS-LRR Gene Family in Arabidopsis thaliana

OBJECTIVE]A full set of disease resistance candidate genes encoding NBS-LRR in a complete ge-nome of Arabidopsis thaliana were analyzed. METHOD] Structural diversities, physical positions, and phylo-genetic relationship were studied by TAIR and Pfam web, database, Chromosome Map Tool, MEGA 3.0 and ClustalX software. RESULTS] 204 genes containing NBS domain were found in genome and most of those were located in 1 and 5 chromosome. The NBS-LRR family genes on different chromosomes exist in the form of monogenic gene or gene elusters, and 71.6% of the NBS-LRR genes were located in gene clusters. That showed there are much copy phenomenons in the evolution of disease resistance genes of Arabidopsis thaliana.