Tumor staging in a Beagle dog with concomitant large B-cell lymphoma and
T-cell acute lymphoblastic leukemia |
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Authors: | Alessandro Ferrari Marzia Cozzi Luca Aresu Valeria Martini |
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Institution: | Clinica Veterinaria San Lorenzo, Suisio, Bergamo, Italy;Veterinary Teaching Hospital, Lodi, Italy;Department of Veterinary Sciences, University of Turin, Grugliasco, TO, Italy;Department of Veterinary Medicine, University of Milan, Lodi, Italy |
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Abstract: | An 8-y-old spayed female Beagle dog was presented with peripheral lymphadenomegaly. Lymph node cytology and flow cytometry led to the diagnosis of large B-cell lymphoma (LBCL). We detected minimal percentages of LBCL cells in peripheral blood and bone marrow samples. However, a monomorphic population of neoplastic cells different from those found in the lymph node was found in the bone marrow. T-cell acute lymphoblastic leukemia was suspected based on flow cytometric immunophenotyping. PCR for antigen receptor rearrangement (PARR) revealed clonal rearrangement of both B-cell and T-cell receptors, and the presence of both neoplastic clones in the lymph node, peripheral blood, and bone marrow. The dog was treated with multi-agent chemotherapy but died 46 d following diagnosis. Tumor staging and patient classification are needed to accurately establish a prognosis and select the most appropriate therapeutic protocol. |
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Keywords: | acute leukemia Beagles flow cytometry lymphoma PARR staging |
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