海岛棉枯萎病抗性与类黄酮代谢相关基因表达量的相关

枯萎病是危害海岛棉生产的重要因素之一, 研究枯萎病抗性分子机制将为培育抗病海岛棉品种、解决枯萎病对海岛棉的危害问题提供坚实的基础。本研究在前期转录组测序的基础上, 对海岛棉枯萎病抗性差异表达基因进行分析(DEG, Differentially Expressed Gene); 以7个抗病性表现不同的海岛棉品种为材料, 利用qRT-PCR的方法研究抗病差异表达基因在不同接菌时间点的表达量差异, 并分析基因表达量与病情指数的相关性。结果表明, DEG分析得出类黄酮代谢通路相关基因与海岛棉枯萎病抗性有关。qRT-PCR分析显示抗病材料中类黄酮代谢通路关键基因的表达量显著高于感病材料。在接菌后多个时间点, 类黄酮代谢通路中的关键基因TT7、CHI和DFR在抗病材料中的表达量显著或极显著高于感病材料, 其中CHI和DFR基因的表达量与病情指数呈显著负相关。综上所述, 类黄酮代谢通路相关基因对海岛棉枯萎病抗性均有影响, 且CHI、TT7和DFR基因是关键基因。

Correlation between Resistance to Fusarium Wilt and Expression of Flavonoid Metabolism Related Genes in Gossypium barbadense L.

Fusarium wilt is one of the important factors that threaten the production of Gossypium barbadense. L. To expanding the molecular mechanism of resistance to Fusarium wilt will provide a solid foundation for cultivating resistant island cotton varieties and solve the problem of wilt disease. In this study DEG(Differentially Expressed Gene) was analyzed on the basis of sequencing of transgenic plants. The Disease resistance differentially expressed genes were analyzed at different inoculation time points of seven Gossypium barbadense. L varieties with different disease resistance levels, and the correlation between gene expression and disease index was analyzed. Flavonoid biosynthetic pathway genes were related to fusarium wilt resistance. qRT-PCR analysis showed that the expression of flavonoid metabolic pathway genes in resistant materials was significantly higher than that in susceptible materials. The expression levels of TT7, CHI and DFR, the key genes in flavonoid metabolic pathways, were significantly higher in resistant materials than in susceptible materials at multiple time points after inoculation, And the expression of CHI and DFR genes was negatively correlated with disease index. In summary, the flavonoid metabolic pathway-related genes have an effect on the resistance to fusarium wilt, among which CHI, TT7 and DFR genes are the key genes.