| 八氢番茄红素合成酶基因(PSY)对大豆的遗传转化 |
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| 引用本文： | 龚学臣,季静,抗艳红,王罡,吴颖,王萍.八氢番茄红素合成酶基因(PSY)对大豆的遗传转化[J].大豆科学,2005,24(1):30-33. |
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| 作者姓名： | 龚学臣 季静 抗艳红 王罡 吴颖 王萍 |
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| 作者单位： | 天津大学农业与生物工程学院,天津,300072;河北北方学院农业科学系,张家口,075131;天津大学农业与生物工程学院,天津,300072;河北北方学院农业科学系,张家口,075131;淮海工学院海洋学院,连运港,222005 |
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| 基金项目： | 国家转基因植物研究和产业化专项基金 |
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| 摘 要： | 以三个大豆品种的无菌苗子叶节为受体,以PSY为目的基因,应用根癌农杆菌介导法对大豆进行了遗传转化,经子叶节丛生芽诱导、茎伸长培养、生根培养和潮霉素选择培养,获得了完整的抗性再生苗,经PCR和PCR-Southern分析鉴定,初步证明外源基因PSY已整合到大豆的基因组中,并对影响遗传转化的因子进行了研究.
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| 关 键 词： | PSY基因 大豆 子叶节 遗传转化 |
| 文章编号： | 1000-9841(2005)01-0030-04 |
GENETIC TRANSFORMATION OF PHYTOENE SYNTHASE GENE( PSY )INTO SOYBEAN |
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| Gong Xuechen,Ji Jing,Kang Yanhong,Wang Gang,Wu Ying,Wang Ping.GENETIC TRANSFORMATION OF PHYTOENE SYNTHASE GENE( PSY )INTO SOYBEAN[J].Soybean Science,2005,24(1):30-33. |
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| Authors: | Gong Xuechen Ji Jing Kang Yanhong Wang Gang Wu Ying Wang Ping |
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| Abstract: | Taking cotyledonary nodes of three varieties of soybean as acceptor, PSY gene were transformed into soybean via agrobacterium-mediated. Hygromycin resistant regeneration plants of soybean were obtained by cotyledonary nodes inducting, striking root cultivation and stem elongation cultivation and hygromycin screening in selective condition. PCR and PCR-Southern analysis from the transferred gene plants proved that PSY has been transferred into genome of soybean. The factors affecting genetic transformation were also investigated. |
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| Keywords: | PSY gene Soybean Cotyledonary node The Genetic transformation |
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