A comparison of the mitogenic effects of a phorbol ester on lymphocytes from bovine spleen,lymph node and peripheral bloodComparaison des effets mitogenes d'un ester de phorbol sur des lymphocytes bovins de rate,de ganglions lymphatiques et de sang peripherique |
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| Authors: | Andrea M Mastro Karen G Pepin |
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| Institution: | Department of Microbiology, Cell Biology, Biochemistry and Biophysics, The Pennsylvania State University, University Park, PA 16802, U.S.A. |
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| Abstract: | A comparison of the mitogenic effects of a phorbol ester on lymphocytes from bovine spleen, lymph node and peripheral blood.Bovine lymphocytes from three tissues, lymph node, spleen and peripheral blood were compared for their mitogenic responses to 12-O-tetraecanoylphorbol-13-acetate (TPA), a phorbol ester tumor promoter. TPA alone was found to be either not mitogenic or caused only a weak response when compared with the mitogenic lectin phytohemagglutinin (PHA). Of the three lymphocyte preparations, blood cells showed the greatest proliferative response to TPA. However, all three, lymph node, blood and spleen cells, showed a co-mitogenic response to TPA. That is, TPA synergistically enhanced DNA synthesis in cells stimulated with a suboptimal concentration of PHA. |
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| Keywords: | phorbol ester lymphocytes mitogenesis comitogenesis bovine lymphocytes phorbol myristate acetate ester de phorbol lymphocytes mitogène comitogène lymphocytes bovins ester de phorbol |
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