3种犬细小病毒感染诊断方法比较

本研究应用犬细小病毒抗原快速检测试剂盒、血凝/血凝抑制试验和PCR方法对120份具有腹泻/呕吐症状的患犬粪便样本进行了犬细小病毒检测。结果显示,3种方法检测阳性份数分别为60,49,68。犬细小病毒抗原快速检测试剂盒与血凝/血凝抑制(HA/HI)试验相比较,敏感性、特异性及总符合率分别为100.0%,84.5%,90.8%;与PCR法相比较,敏感性、特异性及总符合率分别为85.3%,96.2%,90.0%。从试验结果看,HA/HI法具有较高的特异性,但敏感性较低;PCR具有高度敏感性和特异性,但不适合临床推广;细小病毒抗原快速检测试剂盒,在正确操作下结果可靠,是临床诊断犬细小病毒感染的首选方法。

A comparative study of three diagnostic methods for detection of canine parvovirus infection in dogs

Three diagnostic methods for canine parvovirus(CPV) infection were employed to test 120 feces samples collected from dogs with diarrhea and/or vomit in this study.The CPV positive samples were 60,49 and 68,respectively by detecting CPV antigen or nucleic acid using Antigen Rapid CPV Ag Test Kit,haemagglutination/haemagglutination inhibition(HA/HI) and PCR assay.The results of these three methods were evaluated.The percentages showed 100.0%,84.5% and 90.8%,respectively in relative sensitivity,specificity and overall agreement between Antigen Rapid CPV Ag Test Kit and HA/HI,and 85.3%,96.2% and 90.0 %,respectively between Antigen Rapid CPV Ag Test Kit and PCR.These results indicated that HA/HI was more specific than Antigen Rapid CPV Ag Test Kit but had a relatively lower sensitivity.PCR was sensitive and specific,but it was not a suitable test in clinic.The Antigen Rapid CPV Ag Test Kit was the first choice for detecting CPV infection if correctly used.