| 活性污泥驯化过程中芘降解菌的群落多样性分析 |
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| 引用本文： | 周作明,郑娜,方柏山. 活性污泥驯化过程中芘降解菌的群落多样性分析[J]. 湖南农业大学学报(自然科学版), 2009, 35(6): 694-698 |
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| 作者姓名： | 周作明 郑娜 方柏山 |
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| 作者单位： | 华侨大学,福建省高校工业生物技术重点实验室,福建,厦门,361021;华侨大学,福建省高校工业生物技术重点实验室,福建,厦门,361021;华侨大学,福建省高校工业生物技术重点实验室,福建,厦门,361021 |
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| 基金项目： | 国务院侨办自然科学基金项目，福建省自然科学基金项目 |
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| 摘 要： | 将采自炼油厂污水处理生化反应池的活性污泥以芘为唯一碳源进行富集培养与驯化,通过对6个不同时期混合菌群总DNA的提取,采用降落式PCR和变性梯度凝胶电泳(DGGE)技术对细菌16S rDNA 基因V3区进行扩增和产物分离,对活性污泥驯化过程中芘降解菌的群落多样性进行了研究.结果表明,在驯化过程中,芘降解菌的群落结构和种群数量存在明显的演替过程,芘浓度的大小是影响芘降解菌的群落结构和种群数量的重要因素.
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| 关 键 词： | 变性梯度凝胶电泳 芘 降解菌 群落多样性 活性污泥 |
| 收稿时间： | 2009-06-19 |
| 修稿时间： | 2009-09-04 |
Community diversity analysis of pyrene degradable bacteria in the cultivable process of activated sludge |
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| zhou zuoming,zheng na and fang baishan. Community diversity analysis of pyrene degradable bacteria in the cultivable process of activated sludge[J]. Journal of Hunan Agricultural University, 2009, 35(6): 694-698 |
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| Authors: | zhou zuoming zheng na fang baishan |
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| Affiliation: | Huaqiao University,Huaqiao University,xiamen University |
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| Abstract: | The refinery-activated sludge was cultivated with pyrene as one single source of carbon and energy. The community diversity of pyrene degradating strains was studied by extracting the genomic DNA of microbial community in 6 different periods,amplifying the bacterial 16S rDNA V3 fragment with touch-down PCR method,and separating the products by denaturing gradient gel electrophoresis (DGGE). The result showed that there was an obvious syllogistic process in community structure and amount of dominant populations. The concentration of pyrene was a main factor to influence the community diversity of pyrene degradable strains. |
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| Keywords: | denaturing gradient gel electrophoresis pyrene degradating strains community diversity activated sludge |
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