鸡传染性支气管炎H120、W93株活疫苗生产工艺的改进

分别以鸡传染性支气管炎病毒(In-fectiousbronchitisvirus,IBV)H120株、W93株不同病毒量接种10、12日龄SPF鸡胚,并改变后孵化温度生产抗原液,接种后不同时间收获胚液,根据鸡胚的早死率以及胚液的收获量、病毒滴度,选择最佳的生产工艺。结果表明,IBVH120株、W93株以102.7EID50/0.1mL的病毒量接种12日龄鸡胚尿囊腔,在34.5℃孵育35h,可较为显著地降低早死胚率,提高产毒量,且抗原液病毒滴度符合疫苗制备规程的要求。

Improved Tests on Productive Technology of Avian Infectious Bronchitis H120 and W93 Strain Living Vaccines

10-day and 12-day old Specific pathogen free (SPF) embryos were inoculated with different antigen concentrations of infectious bronchitis virus (IBV) H120 and W93 strains, incubated in different temperature, allantoic fluid were harvested in different post-incubated time and titrated, respectively. The manufactured technology of IB living vaccines were optimized according to numbers of early dead embryo,harvesting yields per embryo and antigenic titer of allantoic fluid. The results showed that the productive technology, with 12 day old SPF embryos inoculated with IBV contained 10~(2.7)EID_(50)/0.1 mL antigen concentration,and incubated 35 hours at 34.5 ,was better than productive technology previously. It greatly deduced the numbers of early dead embryo,increased harvesting yields from 8.37 mL to 12.4 mL per embryo.Meanwhile, the antigenic titer was enhanced to more than 10~(6.6)EID_(50)/0.1 mL.