| 烟草内生细菌Itb57的鉴定及其对烟草黑胫病的防治效果 |
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| 引用本文： | 马冠华,周常勇,肖崇刚,陈国康,易龙. 烟草内生细菌Itb57的鉴定及其对烟草黑胫病的防治效果[J]. 植物保护学报, 2010, 37(2): 148-152 |
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| 作者姓名： | 马冠华 周常勇 肖崇刚 陈国康 易龙 |
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| 作者单位： | 1. 西南大学植物保护学院,重庆,400716
2. 西南大学植物保护学院,重庆,400716;中国农业科学院柑桔研究所,重庆,400712 |
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| 基金项目： | 重庆市自然科学基金(2007BB1348,2009BB1294)资助 |
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| 摘 要： | Itb57是一株分离自健康烟草植株的内生细菌,室内测定表明,该菌株对烟草黑胫病菌Phytophthora nicotianae具有很强的拮抗作用.为了进一步明确其分类地位和生物防治潜力,结合形态、生理生化特性及16S rDNA序列(GenBank登录号GQ153538)比对分析,鉴定Itb57菌株为枯草芽孢杆菌Bacillus subtilis;浸种和灌根接种测定结果显示,Itb57可定殖在烟草植株体内,且在不同部位的含菌量不同,以根部最高(3.72×104cfu/g FW),茎部次之,叶片中最少;Itb57发酵液对烟草黑胫病的温室防治效果为69.28%;2007和2008年的田间小区防治效果分别为61.25%和72.49%,与对照化学药剂58%甲霜·锰锌可湿性粉刺的防治效果无显著差异(P>0.05).
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| 关 键 词： | 内生细菌 烟草黑胫病菌 枯草芽孢杆菌 病害生物防治 |
| 收稿时间： | 2009-08-02 |
Identification of the endophytic bacterial isolate Itb57 from tobacco plant and its control efficacy on tobacco black shank caused byPhytophthora nicotianae |
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| Ma Guanhu,Zhou Changyong,Xiao Chonggan,Chen Guokang and Yi Long. Identification of the endophytic bacterial isolate Itb57 from tobacco plant and its control efficacy on tobacco black shank caused byPhytophthora nicotianae[J]. Acta Phytophylacica Sinica, 2010, 37(2): 148-152 |
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| Authors: | Ma Guanhu Zhou Changyong Xiao Chonggan Chen Guokang Yi Long |
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| Affiliation: | College of Plant Protection, Southwest University, Chongqing 400716, China;College of Plant Protection, Southwest University, Chongqing 400716, China; Citrus Research Institute, Chinese Academy of Agricultural Sciences, Chongqing 400712, China;College of Plant Protection, Southwest University, Chongqing 400716, China;College of Plant Protection, Southwest University, Chongqing 400716, China;College of Plant Protection, Southwest University, Chongqing 400716, China |
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| Abstract: | |
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| Keywords: | endophytic bacteria Phytophthora nicotianae Bacillus subtilis disease biological control |
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