QuEChERs-LC-MS/MS快速分析稻谷中12种农药残留方法初探

采用乙腈作为提取剂,通过QuEChERs方法净化,LC-MS/MS检测稻谷中12种农药残留量。通过对比选择50 mg无水硫酸镁、50 mg PSA、20 mg C18作为QuEChERs净化方法的吸附剂,采用0.1%甲酸和2 mmol/L乙酸铵混合水溶液和乙腈作为流动相,LC-MS/MS检测。结果表明,目标化合物线性范围良好,R0.99,加标回收率为70.3%~118.5%,相对偏差小于20.0%。可见,该方法不仅操作简单,成本低,效率高,还能最大限度减少有机溶剂的使用,环保节能,能满足稻谷中农药残留的检测以及确证工作。

Preliminary Study on QuEChERS-LC-MS/MS Method for Rapid Detection of 12 Kinds of Pesticide Residues in Rice

To detect 12 kinds of pesticide in rice by QuEChERS-LC-MS/MS with acetonitrile as extracting agent. By comparison ,the anhydrous magnesium sulfate, PSA, and C18 were selected as adsorbent, and the amount of the three adsorbent was determined as 50 mg, 50 mg and 20 mg. Extraction of rice after purification were detected by LC-MS/MS with 0.1% formic acid and tendency for 2 mmol/L ammonium acetate mixed aqueous solution and acetonitrile as mobile phase. The results showed that a good linear relationship (R>0.99), the recoveries of 12 kinds of pesticide were 70.3%~118.5%, with the precision below 20%. This method not only could meet the inspection work of rice residue but also have the characteristics of energy saving and environmental protection, simple opera-tion and cost saving and high efficiency.