| 人工合成甘蓝型油菜减数分裂中的染色体行为观察 |
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| 引用本文： | 李俊,方小平,罗莉霞,李均,胡琼,李光明,张春雷.人工合成甘蓝型油菜减数分裂中的染色体行为观察[J].江西农业学报,2010,22(1):1-4. |
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| 作者姓名： | 李俊 方小平 罗莉霞 李均 胡琼 李光明 张春雷 |
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| 作者单位： | 中国农业科学院,油料作物研究所,湖北,武汉,430062 |
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| 基金项目： | 国家自然科学基金项目(30270791、30571100);;“十一五”国家科技支撑计划课题(2006BAD21B03) |
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| 摘 要： | 以白菜(Brassica rapaL.,2n=20,AA)为母本,芥蓝(B.oleraceaL.,2n=18,CC)为父本,通过种间杂交、子房培养和染色体加倍,获得新合成甘蓝型油菜(B.napusL.,2n=38,AACC)株系35个。细胞学观察结果表明,新合成甘蓝型油菜的花粉母细胞(PMCs)内染色体在早双线期聚集成两个染色体群,具有较为明显的界限;随后逐渐形成单个二价体,直至终变期产生全部19个二价体,或一些四价体和二价体;在个别PMCs内19个二价体呈9∶10的分开排列;在后期Ⅰ/末期Ⅰ以及第二次减数分裂过程中,染色体分离较为正常。在讨论中提出甘蓝型油菜的染色体在长期的进化过程中所产生的结构变异可能主要来自于A、C基因组内部染色体间交换的假说。
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| 关 键 词： | 人工合成油菜 染色体行为 减数分裂 |
Observation on Chromosome Behavior during Meiosis of Resynthesized B. napus |
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| LI Jun,FANG Xiao-ping,LUO Li-xia,LI Jun,HU Qiong,LI Guang-ming,ZHANG Chun-lei.Observation on Chromosome Behavior during Meiosis of Resynthesized B. napus[J].Acta Agriculturae Jiangxi,2010,22(1):1-4. |
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| Authors: | LI Jun FANG Xiao-ping LUO Li-xia LI Jun HU Qiong LI Guang-ming ZHANG Chun-lei |
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| Institution: | Institute of Oil Crops/a>;Chinese Academy of Agricultural Sciences/a>;Wuhan 430062/a>;China |
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| Abstract: | Using B.oleracea as paternal plants and B.rapa as maternal plants,35 lines of resynthesized B.napus were obtained by means of ovary culture and chromosome doubling.The cytological observation showed that separation and asynchronous division of two groups of chromosomes existed in early diplonema in most PMCs of resynthesized B.napus and they remained relatively independent with obvious limit.Then they paired each other as bivalents until formed 19 bivalents or some quadrivalents and bivalents;in some PMCs,1... |
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| Keywords: | Resynthesized B napus Chromosome behavior Meiosis |
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