Nuclear transfer in cattle : effect of linoleic acid-albumin on freezing sensitivity of enucleated oocytes |
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Authors: | Hochi S Kato M Ito K Hirabayashi M Ueda M Sekimoto A Nagao Y Kimura K Hanada A |
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Institution: | Faculty of Textile Science and Technology, Shinshu University, Ueda, Nagano, Japan. |
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Abstract: | The effect of linoleic acid-albumin (LAA) supplementation to the media for IVM, enucleation, and activation on the developmental potential of bovine embryos produced by nuclear transfer (NT) into frozen-thawed cytoplasts was investigated. Blastomeres derived from morulae was placed in the perivitelline space of frozen-thawed cytoplasts, which were then fused by a DC pulse. The proportion of fused embryos was similar between groups with and without LAA (87 vs. 90%). The proportion of development to blastocysts of NT embryos derived from the media with LAA (14%) was higher than that without LAA (4%), indicating that LAA treatment of bovine oocytes during IVM, enucleation and activation can improve the ability of such cytoplasts after freezing and thawing to develop into blastocysts after NT. |
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