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| 小桐子SRAP-PCR体系优化与M1代变异植株的分子鉴定 | |
| 引用本文： | 陈敦萍,李凌,沈世华,杨清.小桐子SRAP-PCR体系优化与M1代变异植株的分子鉴定[J].核农学报,2009,23(2):209-213. |
| 作者姓名： | 陈敦萍 李凌 沈世华 杨清 |
| 作者单位： | 1. 西南大学,重庆,400715;中国科学院西双版纳热带植物园,云南,西双版纳,666303 2. 西南大学,重庆,400715 3. 中国科学院植物研究所,北京,100095 4. 中国科学院西双版纳热带植物园,云南,西双版纳,666303 |
| 基金项目： | 中国科学院方向性项目，中国科学院西部之光人才培养项目，国家科技支撑计划 |
| 摘 要： | 首次利用SRAP分子标记手段对筛选出的18株经化学试剂、60Co γ辐射和低能离子注入等诱变处理后的小桐子变异植株进行遗传多样性分析。结果显示，22对SRAP引物一共扩增到266条带，其中54条为多态性条带，多态性比例为20.3%；18株形态变异的小桐子植株，大部分不仅在外部形态上发生了变异，而且在DNA水平上也发生了基因突变。这为今后利用诱变技术培育高产优质抗逆性强的小桐子新品种奠定了实践和理论基础。 |
| 关 键 词： | 小桐子 诱变 变异 SRAP |
| 收稿时间： | 2009-12-31 |
| 修稿时间： | 2009-12-31 |
Optimization of SRAP-PCR System and M1 Mutant Molecular Identification of M1 in Jatropha curcas L. |
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| CHEN Dun-ping,LI Ling,SHEN Shi-hua,YANG Qing.Optimization of SRAP-PCR System and M1 Mutant Molecular Identification of M1 in Jatropha curcas L.[J].Acta Agriculturae Nucleatae Sinica,2009,23(2):209-213. | |
| Authors: | CHEN Dun-ping LI Ling SHEN Shi-hua YANG Qing |
| Institution: | 1.Southwest University;the College of Horticulture and Landscape Architecture;ChongQing 400716;2.Xishuangbanna Tropical Botanic Gardens;the Chinese Academy of Sciences;YunNan;Xishuangbanna 666303;3.Institute of Botany;BeiJing 100095 |
| Abstract: | 18 variant plants from mutagenesis of chemical reagents, 60Co γ-irradiation and low energy ion implantation were used for SRAP markers to identify the genetic variations. 22 pairs of SRAP primers amplified to a total of 266 belts, 54 for the polymorphism of the zone, polymorphism ratio was 20.3%. Analysis showed that the 18 variant plants were not only morphological variation, but most of them were changed at DNA level. It provides an evidence that the mutation breeding could be used for new varieties of Jatropha curcas L. with high-yield, high-quality and strong resistance. |
| Keywords: | SRAP |
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