| HPLC-ELSD法检测大豆卵磷脂流动相的选择 |
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| 引用本文： | 杨文梅,马挺军,贾昌喜.HPLC-ELSD法检测大豆卵磷脂流动相的选择[J].农产品加工．学刊,2012(5):7-9,13. |
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| 作者姓名： | 杨文梅 马挺军 贾昌喜 |
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| 作者单位： | 农产品有害微生物及农残安全检测与控制北京市重点实验室,北京102206;北京农学院食品科学与工程学院,北京102206 |
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| 基金项目： | 北京市农产品加工与贮藏工程重点建设学科项目 |
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| 摘 要： | 采用高效液相色谱仪—蒸发光散色检测器(HPLC-ELSD)对超声波法和超临界法提取的大豆卵磷脂进行检测。采用瑞典Kromasil silica100-5色谱柱,异丙醇—正己烷—水为流动相,对流动相比例进行了优化。结果表明,检测超声波萃取卵磷脂样品流动相最佳配比为:V异丙醇∶V正己烷∶V水=54∶28∶10,在23 min内卵磷脂与样品中其他组分完全分离,峰型良好,检测超临界萃取卵磷脂样品流动相最佳配比为:V异丙醇∶V正己烷∶V水=53∶28∶10,在19 min内卵磷脂与样品中其他组分完全分离,峰型良好。
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| 关 键 词： | 高效液相色谱法 流动相 优化 卵磷脂 |
The Choice of Mobile Phase of Soy Lecithin Detection by HPLC-ELSD |
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| YANG Wen-mei
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MA Ting-jun
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JIA Chang-xi.The Choice of Mobile Phase of Soy Lecithin Detection by HPLC-ELSD[J].Nongchanpin Jlagong.Xuekan,2012(5):7-9,13. |
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| Authors: | YANG Wen-mei MA Ting-jun JIA Chang-xi |
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| Institution: | 1,2(1.Beijing Key Laboratory of Agricultural Product Detection and Control of Spoilage Organisms and Pesticide Residue, Beijing 102206,China;2.Faculty of Food Science and Engineering,Beijing University of Agriculture, Beijing 102206,China) |
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| Abstract: | Using high performance liquid chromatography-evaporation scattered color light detector(HPLC-ELSD) to detect soy lecithin by ultrasonic extraction supercritical CO 2 extraction and Sweden Kromasil silica 100-5 chromatographic column, isopropyl alcohol-hexane-water as mobile phase,and optimize the proportion of mobile phase.The results show that the best mobile phase ratio of lecithin sample by ultrasonic extraction is V(ipa) / V(hexane) / V(water) = 54∶28∶10,lecithin and other components are completely separated in 23 min and the peak type is good;the best mobile phase ratio of lecithin sample by supercritical extraction is V(ipa) / V(hexane) / V(water) = 53∶28∶10,lecithin and other components are completely separated in 19 min and the peak type is good. |
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| Keywords: | high performance liquid chromatographic method mobile phase optimization Lecithin |
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