猪带绦虫虫卵及六钩蚴膜超微结构的观察

【目的】观察猪带绦虫虫卵和六钩蚴膜的超微结构。【方法】猪带绦虫病患者用槟榔、南瓜籽法驱虫,收集成熟虫卵。次氯酸钠法破壳后,等渗percoll溶液收集六钩蚴,人工肠液激活六钩蚴。扫描电镜和透射电镜观察虫卵和六钩蚴。【结果】猪带绦虫成熟虫卵呈卵圆形,直径约为50-58μm,由胚膜、内胞质层、六钩蚴膜和六钩蚴等几部分组成;发育期猪带绦虫六钩蚴的膜结构由外向内分别是外胞膜残留层、外胞质层(包括不规则较致密的外层和均质透明的内层)、胚膜(包括胚层和内胞质层)和六钩蚴膜,而成熟六钩蚴的膜结构仅六钩蚴膜一层,厚度约为49-51nm。【结论】猪带绦虫虫卵和六钩蚴膜的超微结构和缩小膜壳绦虫(Hymenolepis diminuta)及肥胖带状绦虫(T.saginata)的超微结构相似,但膜下层的糖原颗粒外周无包膜。

The Ultrastructure of Taenia solium Oncosphere Membrane and Egg

Objective]The ultrastructure of oncosphere membrane and egg of Taenia solium were observed in a study.Method] The decoction areca and pumpkin seeds were taken by the patients (limosis) to get T. Solium mature eggs. After being treated with sodium hypochlorite to break the eggshells, the oncospheres were gathered with isoosmotic percoll solution and were treated with the artificial intestinal juice to activate oncospheres. Then the ultrastructure of egg and oncosphere membrane were observed by scanning electron microscope (SEM) and transmission electron microscope (TEM). Result] The egg was in oval shape with the size of 50-58 μm and consist of embryophore, inner cytoplasmic layer, oncosphere membrane and oncosphere. The structure of developmental T. Solium oncosphere membrane was composed of residual layer of outer envelope, outer cytoplasmic layer (including the outer irregular compact layer and the inner homogeneous lucent layer), the embryophore (including blastoderm layer and inner cytoplasmic layer) and oncosphere membrane from outside to inside, respectively. However, there was only one layer in the mature oncosphere membrane with a size of 49-51 nm. Conclusion] The ultrastructure of the oncosphere membrane and egg of T. Solium are similar to that of Hymenolepis diminuta and T. Saginata except that the envelope is not present around the glycogen granule in underlayer of oncosphere membrane of T. Solium.