紫香无核葡萄胚挽救技术研究

[目的]探讨紫香无核葡萄适宜接种时期及培养基。[方法]以紫香无核葡萄为母本,红提、克瑞森、弗雷无核为父本杂交,另外选择紫香无核自交,对杂交和自交胚珠进行培养挽救。[结果]在授粉55 d后接种,4个组合的胚珠均达到最大发育率和萌芽率,胚珠发育阶段采用ER+半胱氨酸盐酸盐1.0 g/L+水解酪蛋白0.4 g/L+活性炭3.0 g/L培养基发育率最高。[结论]该研究可为进一步无核葡萄杂交育种奠定基础。

Study on Fragrant Zixiang Seedless Grape Embryo Rescue Technique

[Objective ] The aim was to discuss the optimized inoculation period and media of Zixiang seedless grapes. [ Method ] The hybrid was conducted by taking Zixiang seedless grapes as female, and Red, Crimsdn, Frey seedless as male were chose, as well as the selfing among Zixiang purple seedless was carried out, the ovules of hybrid and selfing were cultured and saved. [ Result ] That inoculation after pollination 55 d, the ovules of four combinations reached the maximum germination rate and growth rate, and the growth rate was the highest that the o-vules were cultured by ER ＋ cysteine hydrochlorlde 1.0 g/L ＋ hydrolyzed casein 0.4 g/L ＋ activated carbon 3.0 g/L at ovule developmen-tal stage.