| 核桃离体繁殖技术 |
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| 引用本文： | 吕守芳,闫爱玲,吴燕民,奚声珂.核桃离体繁殖技术[J].经济林研究,2004,22(1):12-14. |
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| 作者姓名： | 吕守芳 闫爱玲 吴燕民 奚声珂 |
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| 作者单位： | 中国林科院林业所,北京,100091;北京市农林科学院林果所,北京,100093;中国农科院生物技术中心,北京,100081 |
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| 基金项目： | 国家林业局“核桃引种研究”课题的部分内容，北京市青年科技骨干培养基金资助项目“核桃品种离体繁殖技术的研究”。 |
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| 摘 要： | 对核桃Juglans regia L.辽核1号、辽核4号、元丰3个品种的茎段进行诱导芽分化、生长和生根成苗试验。筛选出茎段离体初代培养基为DKW BA1．0mg／L；继代培养和壮苗培养基为DKW BA1．0mg／L IBA0．01mg／L．诱导生根培养基为1／2DKW IBA5mg／L,加活性碳1mg／L和问苯三酚1mg／L．生根率达95％以上,重复性稳定。
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| 关 键 词： | 核桃品种 离体繁殖 生根诱导 |
| 文章编号： | 1003-8981(2004)01-0012-03 |
| 修稿时间： | 2003年11月25 |
Micropropagation Techniques for Three Walnut Cultivars |
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| LV Shou-Fang,YAN Ai-Ling,WU Yan-Min,XI Sheng-Ke.Micropropagation Techniques for Three Walnut Cultivars[J].Economic Forest Researches,2004,22(1):12-14. |
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| Authors: | LV Shou-Fang YAN Ai-Ling WU Yan-Min XI Sheng-Ke |
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| Institution: | LV Shou-Fang~1,YAN Ai-Ling~2,WU Yan-Min~3,XI Sheng-Ke~1 |
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| Abstract: | Three walnut cultivars of 'Liaohe 1', 'Liaohe 4', and 'Yuanfeng' were propagated in vitro. Stem fragments were used for bud differentiation, bud growth, and plantlet rooting. Explantlets were initiated on basal DKW with hormone (BA 1.0 mg/L). The new shoots that had emerged from the primary buds were successively transferred to DKW with hormones (BA 1.0 mg/L and IBA 0.01 mg/L). The rooting medium was 1/2 DKW with hormone (IBA 5 mg/L) plus active carbon at 1.0 mg/L and phloroglucinol at 1.0 mg/L, and the rooting percentage exceeded 95 percent with stable repetitiveness. |
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| Keywords: | walnut cultivar in vitro propagation rooting inducement |
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