采用高效液相色谱技术分析茶树体内维生素B6

采用高效液相色谱技术对茶树叶片等组织中维生素B₆（VB₆）的存在形态进行了分析。结果表明：叶片、嫩茎和根中VB₆的含量依次为3.36、1.27、0.18 μg/g鲜重;叶片组织中各型VB₆的构成比为：吡哆醇21%、吡哆醛2.4%、吡哆胺19.3%、磷酸吡哆醛40.8%和磷酸吡哆胺17.9%;叶片中VB₆的含量随叶片的成熟而增加,因叶老化而降低。

Analysis of Vitamin B_6 Derivatives in Tea Plant with High Performance Liquid Chromatography

The determination of Vitamin B6 (VB6) derivated in tea plant was described by using high performance liquid chromatography with fluorescence detector. The results indicated that, the contents of VB6 in tea leaves, tender stem and roots are 3.36 μg/g, 1.27 μg/g and 0.18 μg/g fresh weight, respectively. The constituent ratio of VB6 derivatives are as follows: pyridoxine 21%, pyridoxal 2.4%, pyridoxamine 19.3%, pyridoxal 5′-phosphate 40.8% and pyridoxamine 5′-phosphate 17.9%. The content of VB6 in tea leaves increased with maturity of leaf, and decreased with aging of leaf.