烟草木质素合成途径几个中间代谢物HPLC分析

该文建立了烟草木质素生物合成途径几个中间代谢物咖啡酸（CA）、4 香豆酸（PA）和阿魏酸（FA）的RP-HPLC分析方法.用ODS-C18色谱柱分离、紫外波长扫描检测器检测，并确定320 nm为最佳检测波长；Ｖ（甲醇）∶V(水)（含1.5％乙酸）为20∶80，是最理想的流动相.该方法对CA、PA和FA的回收率分别为95.59%、93.67%、97.32%，相关系数（Rsup]]2/sup]]）分别为0.994 6、0.994 6、0.994 5，满足定量分析要求.经测定，发现转基因烟草CA、PA和FA含量分别为未转化烟草的2.6～3.0、3.4～4.1和7.1～8.2倍. 

HPLC analysis of metabolites in the biosynthetic pathway of tabacco lignin

A method of RP-HPLC with a ODS-C18 column and a UV detector was developed to separate and detect three metabolites such as the caffic acid (CA),4-conmaric acid (PA) and ferulic acid (FA), in the biosynthetic pathway of tobacco lignin. The optimum separation conditions were suggested with the mobile phase containing 1.5% acetic acid （V (methanol)∶V（water）=20∶80） and flow rate 1.0 mL/min and the UV detection wavelength of 320 nm.The recovery rates of CA, PA and FA were 95.59%, 93.67% and 97.32%, and the linear correlation coefficients were 0.994 6, 0.994 6 and 0.994 5 respectively. The results showed that the method of RP-HPLC was suitable for quantitative analysis of the three metabolites. The contents of CA, PA and FA in transgenic tobacco plants were 2.6-3.0,3.4-4.1 and 7.1-8.2 fold higher than those in wild type tobacco plants.