Histological localization assays for diflubenzuron in Pieris brassicae larva (Lepidoptera), by secondary ion microanalysis |
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Authors: | Michel Truchet Sylvie Lauverjat Michel Lamy Jean Claude Denneulin |
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Affiliation: | 1. Laboratoire d''Histophysiologie Fondamentale et Appliquée, ERA, CNRS 570, Université Pierre et Marie Curie, 12 rue Cuvier, 75005 Paris, France;2. Laboratoire de Neuroendocrinologie, Université de Bordeaux I. Avenue des Facultés, 33405 Talence, France |
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Abstract: | No histochemical reaction is available to localize diflubenzuron in tissues. The insecticide can be localized in Pieris tissues by its fluoride atoms, used as tracers in secondary ion microanalysis, which is very sensitive for such light elements. In diflubenzuron-treated insects, especially in females, fluoride intensities are much higher than in controls; images demonstrate a wide distribution of the insecticide in the insect tissues. Thus, besides target organs, i.e., epidermis, diflubenzuron is present in other tissues, such as fat bodies, which may be related to the general metabolic effects and to a detoxification of the insecticide. |
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