东北林蛙烂趾病病原菌的分离鉴定及药敏试验

对东北林蛙烂趾病病原菌进行分离鉴定，并对其药物敏感性进行研究。结果表明：从东北林蛙患病趾处分离到1株溶血性致病茵，经16SrDNA序列分析法鉴定为嗜水气单胞菌。通过对23种抗生素的药敏试验表明，此菌对氟喹诺酮类（如环丙沙星、培氟沙星、诺氟沙星），部分头孢类抗生素（如头孢呋辛、头孢噻肟、头孢吡肟），β-内酰胺类抗生素（如亚胺培南、氨曲南）高度敏感；对部分头孢类抗生素（如头孢噻吩、头孢西丁、头孢他啶），氨基糖苷类抗生素（如卡那霉素、庆大霉素、链霉素）中度敏感；对青霉素类如（青霉素、苯唑西林、氨苄西林、阿莫西林）有耐药性。说明可以考虑用氟喹诺酮类、头孢类或β-内酰胺类抗生素防治东北林蛙烂趾病。

Isolation and identification of pathogenic bacteria from Rana dybowskii with toe rot disease and the drug sensitivity testing

The pathogenic bacteria was isolated and identified from Rana dybowskii with toe rot disease, and then the drug sensitivity testing was carried out. The results showed that a hemolytic pathogen was isolated from the affected toes of Rana dybowskii with toe rot disease ; it was iden- tified as Aeromonas hydrophila by 16S rDNA sequence analysis. The drug susceptibility testing for 23 kinds of antibiotics showed that the Aero- monas hydrophila was highly sensitive to fluoroquinolones （such as ciprofloxacin, pefloxacin,and norfloxacin）, some cephalosporins （such as cefuroxime, cefotaxime, and cefepime） and β-lactam antibiotics （such as imipenem, and aztreonam） ; moderately sensitive to some cephalo- sporins （such as cephalothin, cefoxitin,and ceftazidime） and aminoglycoside antibiotics （such as kanamycin prime, gentamicin, and strepto- mycin） ; resistance to penicillin （such as penicillin, oxacillin, ampicillin, and amoxicillin）. The results indicate that fluoroquinolones, cepha- losporins or β-lactam antibiotics may be considered to prevent the toe rot disease in Rana dybowskii.