山核桃APETALA1同源基因的克隆与序列分析

根据植物花分生组织及花器官形成过程中起重要作用的APETALA1（AP1）基因的高度保守区序列，设计合成一对长度为23bp的聚合酶链式反应（PCR）引物，以山核桃Carya cathayensis因组DNA为模板，采用PCR方法扩增出长为486bp的DNA片段，克隆到pMD18-T载体。测序和序列分析结果表明，获得了山核桃AP1同源基因中的1个片段，该片段序列包含2个内含子，长度分别为86bp和291bp，编码区共编码36个氨基酸。其序列已在Gen荷Bank中注册（注册号为EU155118），在Gen Bank中进行同源性检索结果表明，其氨基酸序列与其他植物AP1同源基因的氨基酸序列同源性高达69％～88％，推测它们在功能上也是相似的。

Cloning and analysis of the APETALA 1 (AP1) homolog gene from Carya cathayensis

From Carya cathayensis, 23 base pair （bp） primers designed according to a conserved sequence of the A PETALA1 （AP1） gene, which has an important function in floral development, were used to amplify a 486 bp fragment by polymerase chain reaction（PCR）. The fragment was cloned into a pMD18-T vector and then sequqnced. Next, the deduced amino acid sequence of the fragment was blasted with A P1 homologous genes of other 17 plants. Results of the 486 bp fragment cloned into a pMD18-T vector produced a fragment Of the A P1 homologous gene： the CcAP1 gene. Also, the sequence analysis indicated two introns of 86 bp and 291 bp in the fragment with the exons encoding 36 amino acids. Afterward, when the CcAP1 gene was blasted with AP1 homologous genes, homology reached 69% - 88%. This result suggested that the CcAP1 gene, registered in GenBank with the accession number EU155118, might have the same function of floral development as other AP1 homologous genes.