鸡巨型艾美耳球虫单卵囊分离及雏鸡扩增试验

采用饱和食盐水漂浮法收集就诊病死球虫阳性鸡小肠中段内容物中的球虫卵囊,恒温培养至孢子化后,用2%琼脂薄板进行球虫单卵囊分离,分别感染7只5日龄雏鸡,对据形态学鉴定为巨型艾美耳球虫的分离株进行2代单卵囊分离和雏鸡感染,取其后代以每只1.0×10⁴个卵囊感染10只10日龄雏鸡进行卵囊扩增,获得大量纯种巨型艾美耳球虫卵囊。结果表明,刀片切割琼脂薄板单卵囊分离法操作简便,单卵囊感染成功率较高,准确率达100%,适用于纯种卵囊的分离与扩增。

Single Oocyst Separation of Eimeria maxima and Oocyst Amplification by Chicken

Coccidia oocysts existed in the small intestine in a clinical died chicken were collected using saturation saline floatation method. After sporulation of oocysts, single oocyst was separated by 2% agarose. Seven 5-day-old chickens were inoculated with the single oocyst. After two times of passage, Eimeria maxima oocysts identified by morphology were again infected to ten 10-day-old chickens, with 1.0×10⁴ oocysts per bird. Thus, numbers of pure oocysts were obtained. The present result suggested that the single oocyst separating technique that used a blade cutting agar plate was a simple and convenient method, which had a higher success rate of single oocyst infection. Therefore, it is useful to amplify the pure oocysts.