抗氧化剂在公猪精液冷冻保存中的应用

近几年来,在公猪精液冷冻稀释液中添加抗氧化剂以提高冷冻精液质量的研究受到广泛的关注。添加谷胱甘肽、超氧化物歧化酶和过氧化物酶、维生素E、L-半胱氨酸和L-谷氨酰胺、中草药成分等抗氧化剂可以有效地防止氧化损伤,提高解冻后精子活率、精子成活力、质膜与顶体完整性等,进而提高冷冻保存精液的受精能力。作者综述了抗氧化剂的抗精子冷冻损伤的作用机制及不同抗氧化剂的应用效果,并对其在公猪精液冷冻保存中的应用前景进行了展望。

Application of Antioxidant During Boar Semen Cryopreservation

In recent years,the application of antioxidant during the cryopreservation of boar semen in order to improve quality of post-thaw semen attracted considerable research efforts. The data revealed that the effect of supplemented glutathione,superoxide and catalase,alpha-tocopherol,L-cysteine and L-glutamine,Chinese herbal medicine in extender could effectively prevented damage,improved kinematics parameters,protected sperm acrosome integrity and plasma membrane integrity,and increasing the sperm fertilizability after freezed-thawed.This review will contribute to make a better understanding of these antioxidants on the mechanisms of resistance to sperm cryodamage,develop an objective evaluation of effectiveness and estimate the applied prospect during the cryopreservation of boar semen.