| 过氧化物酶UTLIEF电泳对苏丹草、高粱及其杂交种品种的真实性鉴定研究 |
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| 引用本文： | 刘敏轩,王赟文,韩建国.过氧化物酶UTLIEF电泳对苏丹草、高粱及其杂交种品种的真实性鉴定研究[J].草业与畜牧,2007(6):9-12,18. |
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| 作者姓名： | 刘敏轩 王赟文 韩建国 |
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| 作者单位： | 中国农业大学草地研究所,农业部牧草与草坪草种子质检中心,北京100094 |
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| 基金项目： | “十五”国家攻关子课题“牧草种子质量检验、认证技术体系及新麦草良种繁育技术的研究与产业化示范（2004BA528B-2-4）” |
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| 摘 要： | 本试验采用超薄层等电聚焦电泳（UTLIEF），检测了苏丹草、高粱以及高粱苏丹草杂交种3个种共15个品种的幼苗、幼根以及萌发种子和干种子的过氧化物酶同工酶。结果表明：采用pH范围为4—5的载体两性电解质制备凝胶进行电泳得到的过氧化物酶电泳图谱谱带数目多且清晰度高，易于鉴定；萌动种子和干种子的过氧化物酶电泳后没有谱带出现。幼苗幼根均出现一定数目的谱带，其中以14d幼苗的过氧化物酶电泳图谱最适宜用作参试品种的鉴定。
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| 关 键 词： | 过氧化物酶 苏丹草 高粱 高粱苏丹草杂交种 UTLIEF |
| 文章编号： | 1673-8403（2007）06-0009-04 |
| 修稿时间： | 2006-06-05 |
Authenticity Identification of POD from Sorghum bicolor, Sorghum sudanense and their Hybrids by UTLIEF |
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| LIU Min-xuan, WANG Yun-wen, HAN Jian-guo.Authenticity Identification of POD from Sorghum bicolor, Sorghum sudanense and their Hybrids by UTLIEF[J].Pruataculture & Animal Husbandry,2007(6):9-12,18. |
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| Authors: | LIU Min-xuan WANG Yun-wen HAN Jian-guo |
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| Institution: | Institute of Grassland Science, China A gricultural University, 100094, China |
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| Abstract: | The POD of young seedling, young root, budding seed and dry seed of 15 varieties from sorghum bicolor, sorghum sudanens and their hybrids was examined by UTLIEF. The results showed that Ampholyte which pH range was 4-5 was suitable for identification; after electrophoresis budding seeds and dry seeds had not any bands, but the POD of young seedling and young root had some bands at the same time, the POD of young seedling for 14 days of the tested varieties was most effective for identification. |
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| Keywords: | POD Sorghum bicolor Sorghum sudanens Sorghum bicolor× Sorghum sudanens UTLIEF |
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