Proviral Genomic
Variation of EIAV Envelop gp 90 Gene Observed in Experimentally Infected Horse |
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Abstract: | The present experiment was designed to observe
the genetic variation of equine infectious anemia virus (EIAV) envelop gp 90 gene in
infected horse. One horse was infected experimentally with P337-V70 strain and showed no
clinical signs after being infected at twice with the same virus strain. Seventeen
proviral sequences covering principal neutralizing domain (PND) of EIAV gp 90 gene were
obtained from the buffy coat and liver of the horse through PCR amplification and cloning.
Comparative analysis of the sequences revealed that some sequences contained the
nucleotide insertions in the PND region. The insertions might be generated by direct
repeat and strand displacement of sequence segment in their PND gene, showing different
lenghts. |
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