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家蚕抗核型多角体病分子标记筛选的遗传基础
引用本文:陈克平,鲁成,向仲怀,姚勤,李木旺,侯成香.家蚕抗核型多角体病分子标记筛选的遗传基础[J].西南农业大学学报,2001,23(3):196-198,204.
作者姓名:陈克平  鲁成  向仲怀  姚勤  李木旺  侯成香
作者单位:[1]西南农业大学蚕桑丝绸学院农业部蚕桑学重点实验室,重庆400716 [2]中国农业科学院蚕业研究所,江苏镇江212018
基金项目:国家自然科学基金重点项目(39730370)和中国农业科学院“九五”重点课题
摘    要:家蚕对NPV抵抗性主要受一对位于常染色体上显性基因控制,又为不完全显性,Z染色体上有微效修饰基因,有偏父遗传现象存在。根据其遗传规律的特殊性,提出常染色体上NN主基因和性染色体上Z^ Z^ 修饰基因控制假设,并得到试验验证。利用近等基因系寻找抗性分子标记,其交配方式不同,分别可构建NN,Z^ Z^ ,NNZ^ Z^ 3种近等基因系。同时提出F2群体寻找分子标记的交配方式。

关 键 词:家蚕  抗NPV  分子标记  交配设计  抗病性
文章编号:1000-2642(2001)03-0196-03

GENETIC BASIS OF CONSTRUCTING F2 POPULATIONS AND NEAR ISOGENIC LINES OF BmNPV RESISTANCE MOLECULAR MARKERS
CHEN Ke-ping ,LU Cheng ,XIANG Zhong-huai ,Yao Qin ,LI Mu-wang ,HOU Cheng-xiang.GENETIC BASIS OF CONSTRUCTING F2 POPULATIONS AND NEAR ISOGENIC LINES OF BmNPV RESISTANCE MOLECULAR MARKERS[J].Journal of Southwest Agricultural University,2001,23(3):196-198,204.
Authors:CHEN Ke-ping  LU Cheng  XIANG Zhong-huai  Yao Qin  LI Mu-wang  HOU Cheng-xiang
Institution:CHEN Ke-ping 1,LU Cheng 1,XIANG Zhong-huai 1,Yao Qin 2,LI Mu-wang 2,HOU Cheng-xiang 2
Abstract:NPV resistance in silkworm (Bombyx mori) is mainly regulated by a pair of dominant genes on an autosome. It is, however, not complete dominance, because minor modifer genes are present on Chromosome Z and a patroclinal tendency is shown in inheritance. Based on the hereditary properties of NPV resistance, a hypothesis was proposed and confirmed that the resistance is controlled by NN main gene on an autosome and Z Z minor genes on the sex chromosome. By different mating methods, three near isogenic lines, i.e. NN, Z Z , and NN Z Z , which are used in search for molecular markers of resistance, were constructed. A mating model is put forward for checking molecular markers using the F 2 populations.
Keywords:Bombyx mori  NPV resistance  molecular marker  mating system
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