丛枝菌根真菌根内菌丝碱性磷酸酶活性与菌根共生效应的研究

试验研究 3种丛枝菌根真菌根内菌丝碱性磷酸酶活性与菌根共生效应的结果表明 ,3种丛枝菌根真菌对宿主植物的效应不同 ,与接种G .spp处理和未接种对照相比 ,接种G .m和G .i处理显著增加玉米地上部和根系干物质量、P浓度和吸P量 ,但后两者间无显著差异 ;而接种G .spp处理与对照无显著差异。播种后 35d时接种G .m和G .i处理根内菌丝碱性磷酸酶活性显著高于接种G .spp处理 ,而前二者间无显著差异 ,且随生长时间的变化趋势相似 ,35d时酶活性最高 ,35～ 5 0d呈迅速下降趋势 ,至 70d时酶活性仍下降且趋于平缓。G .spp酶活性则一直处于较低水平 ,随生长时间的延长略有起伏。即接种不同丛枝菌根真菌时 ,根内菌丝碱性磷酸酶活性高的菌根真菌对玉米生长促进作用较大 ,可提高玉米P营养状况 ;反之则对玉米生长和P营养状况无明显促进作用 ,且与对照无显著差异。出苗后 35d时根内菌丝碱性磷酸酶活性是预测丛枝菌根真菌对玉米生长效应的有效生理指标之一。

Studies on the relationship between the activity of alkaline phosphatase in intraradical hyphae of arbuscular mycorrhizae fungi and efficiency of mycorrhizal symbiosis

The effects of the activity of alkaline phosphatase in intraradical hyphae of three arbuscular mycorrhizae fungi on the growth of maize are studied in this paper. The results show that G.m and G.i inoculated treatments can significantly improve the dry weight of the maize plants, P concentration and P uptake by maize plants compared with G.spp inoculated treatment. But there is no significant difference between G.m and G.i treatments. At 35 days after sowing(DAS), alkaline phosphatase (ALP) activity in intraradical hyphae of G.m and G.i is significantly higher than that of G.spp, however no significant difference appears between G.m and G.i. The changes of alkaline phosphatase activity of G.m and G.i at the infection age show similar trends. Alkaline phosphatase activity is highest at 35 DAS, but decreases sharply from 35 to 50 DAS and then keeps stable up to 70 DAS. As far as G.spp concerned, there is a little fluctuation of alkaline phosphatase activity at infection age, and the enzyme activity always keeps very low. In other words, when inoculating arbuscular mycorrhizae fungi with high alkaline phosphataes activity (35 DAS), the maize has a great improvement of dry weight and P nutrition. Otherwise, there is no significant effect when the maize is inoculated with arbuscular mycorrhizal fungi of low alkaline phosphatase activity. In conclusion, ALP activity in intraradical hyphae at 35 DAS is a useful physiology marker for predicting AM fungi effect on mycorrhizal plants.