苜蓿内生菌中高效AHL降解菌的筛选和鉴定 |
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引用本文: | 汪玲玲,龚伟伦,刘霭莎. 苜蓿内生菌中高效AHL降解菌的筛选和鉴定[J]. 华南农业大学学报, 2010, 31(2) |
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作者姓名: | 汪玲玲 龚伟伦 刘霭莎 |
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作者单位: | 华南农业大学生命科学学院,广东,广州,510642;华南农业大学生命科学学院,广东,广州,510642;华南农业大学生命科学学院,广东,广州,510642 |
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基金项目: | 华南农业大学校长基金,华南农业大学大学生科技创新活动 |
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摘 要: | 从中国新疆、内蒙古和墨西哥等地分离苜蓿内生菌133株,平板筛选到8株细菌具有N-酰基高丝氨酸内酯(N-Acyl-L-homoserinelactone,AHL)降解活性,其中活性较强的菌株有r-12、r-9和R1.5.对8株AHL降解菌做AHL唯一碳源试验,结果发现菌株S35和N46在液体AHL唯一碳源培养基中生长情况较好,D600 nm分别为0.415和0.386,菌株r-9的生长情况最差,D600 nm为0.015.菌株R1.5还具有耐高温、耐盐碱、能分解无机磷、在无氮培养基上生长等优点.经细菌形态学观察、生理生化试验以及16S rDNA序列分析,初步确定菌株R1.5为1株地衣芽孢杆菌.
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关 键 词: | 苜蓿内生菌 AHL降解菌 筛选 鉴定 |
Screening and Identification of AHL-Degrading Bacteria from Alfalfa Entophytes |
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Abstract: | One hundred and thirty-three alfalfa entophytes were isolated from the roots and stems of alfalfa grown in Xinjiang Province,Inner Mongolia Autonomous Region in China,and Mexico.Eight isolates showed N-Acyl-L-homoserinelactone (AHL) degrading activity,especially strain r-12,r-9,R1.5 showed stronger activity than others by the plate screening.When AHL used as unique carbon and energy,strain S35 (D_(600 nm)=0.386) and N46 (D_(600 nm)=0.415) grew well,but D_(600 nm) of strain r-9 was 0.015.Strain R1.5 grew well under high temperature,high salt concentration and high pH.Strain R1.5 also grew on the medium without additive nitrogen,and dissolved phosphor.Based on phenotype test,physi-biochemical results and 16S rDNA sequence analysis,strain R1.5 was identified as Bacillus licheniformis. |
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Keywords: | alfalfa entophytes AHL-degrading bacteria screen identify |
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