小麦生育酚环化酶基因全长cDNA的克隆与分析

生育酚环化酶(TC)是维生素E生物合成途径中的关键酶。通过RACE-PCR得到了小麦生育酚环化酶基因的cDNA序列,该基因的cDNA全长1 769bp,包含一个长为1 404bp的完整开放读码框,推导的氨基酸序列与水稻、玉米、芝麻、马铃薯、拟南芥的生育酚环化酶基因的一致率分别为91%、88%、71%、68%、67%。系统进化分析结果表明,不同植物来源的生育酚环化酶基因聚为3类。

Cloning and Sequence Analysis of Tocopherol Cyclase cDNA from Wheat (T. Aestivum L.)

Tocopherol cyclase (TC) is a key enzyme for plant vitamin E biosynthesis. The cDNA of wheat TC was cloned by RACE-PCR with primers designed based on a EST (BQ161011) sequence. The full-length cDNA sequence contained a complete open reading frame (ORF) of 1404 bp, which encoding 467 amino acid residues. Homology analysis of the deduced amino acid showed 91%, 88%, 71%, 68% and 67% identity with TC from rice, maize, sesame, potato and Arabidopsis thaliana, respectively. The result of phylogenetic analysis indicated that various TC sequences were clustered into three groups.