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犬细小病毒北京分离株VP2基因的表达与抗原性分析
引用本文:曾妮,李刚,朱鸿飞,侯绍华,史利军.犬细小病毒北京分离株VP2基因的表达与抗原性分析[J].中国畜牧兽医,2010,37(4):185-186.
作者姓名:曾妮  李刚  朱鸿飞  侯绍华  史利军
作者单位:(中国农业科学院北京畜牧兽医研究所, 北京 100193)
摘    要:本研究基于犬细小病毒(canine parvovirus, CPV) VP2基因序列设计引物,利用PCR技术扩增该基因。扩增的VP2基因连入表达载体pET-32a(+),经PCR及双酶切鉴定基因连入正确。重组表达载体转入大肠杆菌后,经IPTG诱导,SDS-PAGE及Western blotting分析表明有特异蛋白的表达,分子质量大小为87 ku。将表达的蛋白包被酶标板后用抗犬细小病毒单抗与其反应,表明表达的VP2蛋白可以与相应抗体特异结合。

关 键 词:犬细小病毒  表达  抗原性  

Prokaryotic Expression and Analysing Antigenicity of VP2 Gene Protein of CPV
ZENG Ni,LI Gang,ZHU Hong-fei,HOU Shao-hua,SHI Li-jun.Prokaryotic Expression and Analysing Antigenicity of VP2 Gene Protein of CPV[J].China Animal Husbandry & Veterinary Medicine,2010,37(4):185-186.
Authors:ZENG Ni  LI Gang  ZHU Hong-fei  HOU Shao-hua  SHI Li-jun
Institution:(Institute of Animal Science and Veterinary Medicine, Chinese Academy of Agricultural Sciences, Beijing 100193, China)
Abstract:One specific pairs of primers, which were designed by canine parvovirus(CPV) VP2 gene sequence, were used to clone the VP2 gene isolated from Beijing through PCR. VP2 gene was subcloned from pEASY-T-VP2 into prokaryotic expression vector pET-32a. Sequence analysis confirmed that the VP2 gene was inserted correctly. SDS-PAGE and Western-blotting analysis revealed that the VP2 protein was expressed at high level in Escherichia coli. Through ELISA assay, the expressed VP2 protein has fine immunogenicity.
Keywords:canine parvovirus  expression  immunogenicity
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