| 鸵鸟种质资源保存中的体细胞培养 |
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| 引用本文： | 袁建霞,方小潮,张劳.鸵鸟种质资源保存中的体细胞培养[J].中国畜牧杂志,2004,40(7):8-11. |
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| 作者姓名： | 袁建霞 方小潮 张劳 |
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| 作者单位： | 1. 中国农业大学动物科技学院,北京,100094
2. 广东江门金鸵产业发展有限公司,广东江门,529000 |
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| 摘 要： | 本研究利用皮肤植块法建立了鸵鸟胚胎成纤维细胞 ,并进行了传代、冻存、复苏和染色体核型分析实验。传代实验和染色体核型分析实验表明 ,鸵鸟胚胎成纤维细胞系可在体外存活 7～ 8代 ,且保持核型正常 ,而且 ,细胞冻存和复苏对核型也没有显著影响 ,从而证明利用体外培养的体细胞是进行保种可行的。
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| 关 键 词： | 畜牧学 细胞培养 种质资源保存 鸵鸟 |
| 文章编号： | 0258-7033(2004)07-0008-03 |
| 修稿时间： | 2003年12月20 |
Somatic Cell Culture in Ostrich Conservation |
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| YUAN Jian-xia,FANG Xiao-chao,ZHANG Lao.Somatic Cell Culture in Ostrich Conservation[J].Chinese Journal of Animal Science,2004,40(7):8-11. |
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| Authors: | YUAN Jian-xia FANG Xiao-chao ZHANG Lao |
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| Institution: | YUAN Jian-xia~1,FANG Xiao-chao~2,ZHANG Lao~ |
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| Abstract: | Ostrich embryo fibroblast cells were cultured by skin explant technique. Subculture, freezing, thawing andchromosome karyotyping of cells were processed. Experiment of subculture and chromosome karyotype indicated that ostrich embryo fibroblast cells could grow through 7～8 population doublings with normal karyotypes. In addition, freezing and thawing didn't influence karyotype significantly. Therefore ostrich conservation is practicable by somatic cell culture in vitro. |
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| Keywords: | Animal science Somatic cell culture Ostrich conservation Ostrich |
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