| 藏獒线粒体DNA D-loop区序列测定及其分类地位研究 |
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| 引用本文： | 李隐侠,李齐发,刘振山,赵兴波,潘增祥,孙源荣,徐银学,谢庄. 藏獒线粒体DNA D-loop区序列测定及其分类地位研究[J]. 南京农业大学学报, 2009, 32(2) |
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| 作者姓名： | 李隐侠 李齐发 刘振山 赵兴波 潘增祥 孙源荣 徐银学 谢庄 |
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| 作者单位： | 1. 南京农业大学动物科技学院动物生殖遗传与分子进化实验室,江苏,南京,210095
2. 中国农业大学农业生物技术国家重点实验室,北京,100094 |
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| 基金项目： | 江苏省自然科学基金，中国农业大学-南京农业大学青年教师开放科研基金 |
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| 摘 要： | 根据家犬线粒体基因组DNA序列设计引物,获得了藏獒(Tibetan mastiff)线粒体DNA D-loop区全序列,并以灰狼、郊狼作为外类群对藏獒、家犬亚种内6个大型家犬品种进行了系统发育分析.试验结果发现:藏獒线粒体DNA D-loop区序列全长1 250 bp,与家犬源序列的同源性为96.06%,其中有20个碱基缺失;系统发育分析发现藏獒、家犬亚种内6个大型家犬品种与灰狼聚为一大类,而郊狼自成一类,说明藏獒与其他家犬品种一样起源于灰狼,是家犬亚种内的一个品种,在分类地位上隶属于食肉目、犬科、犬属、狼种、家犬亚种;在家犬亚种内,藏獒与英国牧羊犬、兰伯格犬聚为一类,而德国牧羊犬与瑞典猎鹿犬、黑俄罗斯更犬聚在一起,再与圣伯纳犬聚为一类,从分子水平上验证了英国牧羊犬等大型犬可能含有藏獒血统的观点.
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| 关 键 词： | 藏獒 线粒体DNA D-loop区 系统发育关系 起源 分类地位 |
Sequencing D-loop of mitochondrial DNA in Tibetan mastiff and study on taxonomic status |
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| LI Yin-xia,LI Qi-fa,LIU Zhen-shan,ZHAO Xing-bo,PAN Zeng-xiang,SUN Yuan-rong,XU Yin-xue,XIE Zhuang. Sequencing D-loop of mitochondrial DNA in Tibetan mastiff and study on taxonomic status[J]. Journal of Nanjing Agricultural University, 2009, 32(2) |
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| Authors: | LI Yin-xia LI Qi-fa LIU Zhen-shan ZHAO Xing-bo PAN Zeng-xiang SUN Yuan-rong XU Yin-xue XIE Zhuang |
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| Affiliation: | 1.Laboratory of Animal Reproductive Genetics and Molecular Evolution;College of Animal Science and Technology;Nanjing Agricultural University;Nanjing 210095;China;2.State Key Laboratory for Agribiotechnology;China Agricultural University;Beijing 100094;China |
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| Abstract: | In this study,the primers for D-loop of Tibetan mastiff were designed according to the mitochondrial genome sequence of domestic dog,and the phylogenetic trees of Tibetan mastiff and 6 representative breeds of large breed dogs were constructed using grey wolf and coyote as outgroups.The results showed that,the D-loop region of Tibetan mastiff was 1 250 bp,owning 96.06% homology of the domestic dog D-loop sequence,there existed 20 bp deficiency.Phylogenetic analysis found that Tibetan mastiff,6 domestic dog ... |
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| Keywords: | Tibetan mastiff mitochondrial DNA D-loop region phylogenetic relationship origin taxonomic status |
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