湖羊排卵相关基因表达水平与繁殖性能关系的研究

 【目的】研究湖羊卵巢组织COX2、HAS2、TSG6和PTX3的mRNA表达水平与湖羊繁殖性能的相关性,筛选影响湖羊高产的候选基因,为揭示湖羊高繁多胎分子遗传机理提供依据。【方法】选取16只经产湖羊母羊,分为低产组和高产组,于发情后24—36 h屠宰,应用RT-PCR技术检测COX2、HAS2、TSG6和PTX3的组织表达特征,进一步利用实时荧光定量PCR技术分析各基因mRNA在低产组和高产组湖羊卵巢组织中的表达差异。【结果】COX2、HAS2、TSG6和PTX3均在湖羊卵巢组织中表达,在卵巢组织,高产组TSG6和PTX3的mRNA表达极显著高于低产组(P＜0.01),低产组HAS2的mRNA表达极显著高于高产组(P＜0.01),而COX2的mRNA表达在高产组和低产组间无显著差异(P＞0.05)。【结论】HAS2、TSG6和PTX3的mRNA表达在高产组和低产组间差异极显著,可能与湖羊繁殖性能相关,可作为影响湖羊高产的候选基因。

Relationship Between the Reproductive Ability of Hu Sheep and the Expression Level of Genes Correlated with Ovulation

【Objective】 The present study was performed to investigate the relationships between the mRNA level of COX2, HAS2, TSG6 and PTX3 in ovaries and the reproductive ability of Hu Sheep, so as to provide references for finding hyperprolificacy candidate genes and understanding the molecular genetic mechanism of high fecundity in Hu sheep. 【Method】Sixteen adult Hu ewes, classified as high-yielding (H) or low-yiebding group (L), were sacrificed for tissue sampling between 24-36h after estrus diagnosis. The tissue distributions of COX2, HAS2, TSG6 and PTX3 mRNA were detected by RT-PCR and the gene expression levels in the ovaries were investigated by real-time PCR. 【Result】COX2, HAS2, TSG6 and PTX3 all expressed in ovary of Hu sheep. The expression level of COX2 mRNA in the ovary did not differ between H and L group (P＞0.05), but TSG6 and PTX3 mRNA abundance in the ovary was extremely higher in H group than that in L group (P＜0.01). In addition, HAS2 mRNA abundance in the ovary was extremely higher in L group than that in H group (P＜0.01). 【Conclusion】 The results suggested that HAS2, TSG6 and PTX3 might play a key role in reproductive ability of Hu Sheep and might be candidate genes for high fecundity in Hu sheep.