| 草鱼出血病病毒854株的纯化及其理化特性 |
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| 引用本文： | 曾令兵 贺路. 草鱼出血病病毒854株的纯化及其理化特性[J]. 淡水渔业, 1992, 0(2): 3-5 |
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| 作者姓名： | 曾令兵 贺路 |
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| 作者单位： | 中国水产科学研究院长江水产研究所，中国水产科学研究院长江水产研究所 沙市 434000，沙市 434000 |
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| 摘 要： | 离心技术纯化病毒。病毒粒子具双层衣壳,直径约为65nm。病毒对氯仿、乙醚、酸碱、胰蛋白酶的处理不敏感。冻融和56℃、1小时处理,毒力下降显著。-80℃、-30℃两种温度条件下保存,其毒力损失有差异。鱼用消毒剂鱼康有杀病毒作用。
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| 关 键 词： | 草鱼 出血病 病毒 纯化 理化特性 |
STUDIES ON THE PURIFICATION AND PHYSICAL——CHEMICAL CHARACTERISTICS OF THE GRASS CARP HEMORRHAGE VIRUS STRAIN 854 |
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| Abstract: | Grass carp hemorrhage virus strain 854(GCHV-854)was purified by centrifuge techniques and the viral particles are about 65nm in diameter and composed of a double capsid. GCHV-854 is relatively stable to organic solvents chloroform, ether, to acidic or alkaline pH and the trypsinization, but sensitive to freezing——thawing and heat treatment.The decreases in infectivity of GCHV-854 stored at—30℃、—80℃during the same period are apparently different. Yukang-a kind of disinfectan for fish has virucidal effect in experiment. |
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