6-BA、NAA对苜蓿不同外植体愈伤组织培养的影响

以新牧1号和新疆大叶两个苜蓿品种为试材,研究了7个培养基对不同无菌苗外植体愈伤组织诱导和生长影响,以及不同浓度的植物生长调节物质6-苄氨基腺嘌呤(6-BA)和萘乙酸(NAA)对愈伤组织生长及分化的作用。结果表明:(1)浓度在2~8 mg/L,NAA有利于苜蓿愈伤组织的诱导,随浓度的增大呈促进作用,而在1~3 mg/L,1mg/L 6-BA有利于苜蓿愈伤组织的诱导,随浓度的增大呈抑制作用;(2)MS+2,4-D 2mg/L+NAA 4 mg/L+6-BA 1 mg/L即MS4为新牧1号的较佳诱导培养基,MS+2,4-D 2 mg/L+NAA 8 mg/L+6-BA 1 mg/L即MS5为新疆大叶苜蓿的较佳诱导培养基;(3)以无菌苗下胚轴、子叶及叶片为外植体,确定下胚轴为诱导愈伤组织的最佳外植体。

Effects of 6-BA and NAA on Different Explants of Cultured Tissues of Alfalfa

The paper studied effects of seven kinds of medium on induction and growth of callus,using different of explant of Medicago varia.cv.Xinmu No.1 and Medicago sativa L.cv.Xinjiang Daye,and effects of different concentration of 6-BA(6-Benzylaminopurine) and NAA(1-Naphthyacetic Acid) on induction,differentiation callus.The results are as follows:(1) The concentration of 2～8 mg/L,NAA is benefit to induce callus,if it's concentration was higher,the callus of alfalfa were better.The concentration of 1～3 mg/L,1mg/L 6-BA is benefit toinduce callus,if it's concentration was higher,the callus of alfalfa were worse.(2)MS+2,4-D 2 mg/L +NAA 4 mg/L +6-BA 1 mg/L was the better induction medium of Xinmu No.1.MS+2,4-D 2 mg/L +NAA 8 mg/L +6-BA 1 mg/L was the better induction medium of Xinjiang Daye.(3)Using the explant of hypocotyls,cotyledon and leaves as explants,hypocotyls was the best explant of induction callus medium.