烟草野火病菌的快速检测 |
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引用本文: | 刘雅婷,张世珖,李永忠. 烟草野火病菌的快速检测[J]. 西北农林科技大学学报(自然科学版), 2002, 30(6): 138-143 |
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作者姓名: | 刘雅婷 张世珖 李永忠 |
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作者单位: | 1. 云南农业大学,农学与生物技术学院,云南,昆明,650201 2. 云南农业大学,云南省植物病理重点实验室,云南,昆明,650201 3. 云南农业大学,烟草学院,云南,昆明,650201 4. 云南省烟草公司曲靖分公司,云南,曲靖,655001 |
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基金项目: | 云南省烟草公司基金项目(98A55) |
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摘 要: | 对采自云南各大烟区的烟草野火病菌株进行了分离、鉴定,用通过筛选致病力强的烟草野火病菌株为抗原,制备了烟草野火病菌特异性的抗血清,建立了一套快速检测烟草野火病菌的方法,制备了SPA-ELISA反应试剂盒,应用玻片凝集或协同凝集反应与SPA-ELISA反应试剂盒相结合的方法,使检测真正做到了简便、快速、灵敏、准确。
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关 键 词: | 烟草野火病 烟草野火病菌 快速检测 间接-ELISA SPA-ELISA 凝集反应 |
文章编号: | 1671-9387(2002)06-0138-06 |
收稿时间: | 2001-11-01 |
修稿时间: | 2001-11-01 |
Studies on rapid detection of tobacco wildfire (Pseudomonas syringae pv.tabaci) in Yunnan |
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Affiliation: | LIU Ya ting1,ZHANG Shi guang2*,LI Yong zhong3,WANG Shao kun4(1 Faculty of Agricultural Science and Technology;2 Phytopathology Laboratory of Yunnan Province;3 Faculty of Tabacco,Yunnan,Kunming 650201,China;4 Qujing Tobacco Company,Yunnan Provience,Qujing,Yunnan 655001,China) |
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Abstract: | Strains of tobacco wildfire from Yunnan were isolated and identified.Pseudomonas sytingae pv. tabaci specific antiserum was obtained through screening the strong-virulent strains as antigen. Straphyloclcal protein A ELISE(SPA-ELISA) kits were developed successfully and this was the first time to establish the rapod detecting method of the pathogen.Detection techniques become easieer,rapoder,more sensitive and accurate,by combining SPA-ELISA with agglutination test(slide agglutination and coagglutination). |
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Keywords: | tobacco wildfire Pseudomonas syringae pv.tabaci rapid detection indirect-ELISA SPA-ELISA agglutination |
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