| 红螯螯虾血细胞分类、结构与免疫特性研究 |
| |
| 引用本文： | 冼健安,张秀霞,潘训彬,鲁耀鹏,李军涛,段瑞军,郑佩华,王冬梅.红螯螯虾血细胞分类、结构与免疫特性研究[J].水生态学杂志,2019,40(5):84-90. |
| |
| 作者姓名： | 冼健安 张秀霞 潘训彬 鲁耀鹏 李军涛 段瑞军 郑佩华 王冬梅 |
| |
| 作者单位： | 中国热带农业科学院热带生物技术研究所海南省海洋生物资源功能性成分研究与利用重点实验室;华南师范大学生命科学学院广东省水产健康安全养殖重点实验室 |
| |
| 基金项目： | 中国热带农业科学院基本科研业务费专项资金（1630052016011，1630052019013）；海南省重点研发计划项目（ZDYF2016084） |
| |
| 摘 要： | 血细胞在甲壳动物免疫过程中起重要作用,探讨红螯螯虾(Cherax quadricarinatus)不同类型血细胞结构与免疫特性有助于其病害防治。应用流式细胞术(FCM),根据前向角散射光(FSC)和侧向角散射光(SSC)的强度差异对血细胞进行分类,利用特异性荧光染料进行标记,分析血细胞总数(THC)、线粒体数量、溶酶体数量、吞噬活力、活性氧(ROS)含量、一氧化氮(NO)含量和非特异性酯酶活力。结果显示,应用FCM可以区分透明细胞(HC)、半颗粒细胞(SGC)和颗粒细胞(GC)三类血细胞,其占比分别为9.82%、61.11%和25.24%;红螯螯虾平均血细胞总数(THC)为(8.43±0.87)×10~6个/mL;GC含有最多数量的线粒体和溶酶体,HC中含量最少;HC、SGC和GC的吞噬率分别为2.54%、14.45%和6.98%,SGC吞噬活力最强,HC最弱;HC、SGC和GC的活性氧(ROS)含量分别为7.80、45.95和134.69 AU,GC的ROS含量最高,HC最低;HC、SGC和GC的一氧化氮(NO)含量分别为8.20、79.78和344.31 AU,GC的NO含量最高,HC最低;HC、SGC和GC的非特异性酯酶活力分别为86.59、121.84和236.91AU,GC的酯酶活力最高,HC最低。研究表明,红螯螯虾三类血细胞在形态结构、数量及免疫特性上均存在差异,SGC的数量最多、吞噬活力最强,而GC含有最多与能量供应、免疫防御相关的细胞器,并拥有最强的氧化活力和酯酶活力,表明GC和SGC是红螯螯虾免疫防御过程中发挥主要作用的血细胞类型。
|
| 关 键 词： | 红螯螯虾 流式细胞术 血细胞 免疫力 |
| 收稿时间： | 2017/9/30 0:00:00 |
| 修稿时间： | 2019/9/17 0:00:00 |
Classification, Structure and Immunological Characterization of Haemocytes in Red Claw Crayfish, Cherax quadricarinatus |
| |
| XIAN Jian-an,ZHANG Xiu-xi,PAN Xun-bin,LU Yao-peng,LI Jun-tao,DUAN Rui-jun,ZHENG Pei-hua and WANG Dong-mei.Classification, Structure and Immunological Characterization of Haemocytes in Red Claw Crayfish, Cherax quadricarinatus[J].Journal of Hydroecology,2019,40(5):84-90. |
| |
| Authors: | XIAN Jian-an ZHANG Xiu-xi PAN Xun-bin LU Yao-peng LI Jun-tao DUAN Rui-jun ZHENG Pei-hua and WANG Dong-mei |
| |
| Institution: | Hainan Provincial Key Laboratory for Functional Components Research and Utilization of Marine Bio-resources, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, P.R.China;,Hainan Provincial Key Laboratory for Functional Components Research and Utilization of Marine Bio-resources, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, P.R.China;,Guangdong Provincial Key Laboratory for Healthy and Safe Aquaculture, College of Life Science, South China Normal University, Guangzhou 510631, P.R.China,Guangdong Provincial Key Laboratory for Healthy and Safe Aquaculture, College of Life Science, South China Normal University, Guangzhou 510631, P.R.China,Hainan Provincial Key Laboratory for Functional Components Research and Utilization of Marine Bio-resources, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, P.R.China;,Hainan Provincial Key Laboratory for Functional Components Research and Utilization of Marine Bio-resources, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, P.R.China;,Guangdong Provincial Key Laboratory for Healthy and Safe Aquaculture, College of Life Science, South China Normal University, Guangzhou 510631, P.R.China and Hainan Provincial Key Laboratory for Functional Components Research and Utilization of Marine Bio-resources, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, P.R.China; |
| |
| Abstract: | |
| |
| Keywords: | Cherax quadricarinatus flow cytometry haemocyte immunity |
| 本文献已被 CNKI 等数据库收录！ |
| 点击此处可从《水生态学杂志》浏览原始摘要信息 |
| 点击此处可从《水生态学杂志》下载免费的PDF全文 |
|
