Localization of calcium regulatory hormones in fish |
| |
Authors: | Toyoji Kaneko Stephen Harvey Loren W Kline Peter K T Pang |
| |
Institution: | (1) Department of Physiology, University of Alberta, Edmonton, Alberta, T6G 2H7, Canada |
| |
Abstract: | Immunocytochemical localization of hypocalcin, a hypocalcemic factor in the corpuscles of Stannius (CS), in American eels
was examined at the light (ABC method) and electron microscopic (protein A-gold technique) levels with the specific antiserum
raised against purified rainbow trout hypocalcin. Only type 1 cells in the CS were immunoreactive in the light microscopic
immunocytochemistry. At the electron microscopic level, however, hypocalcin immunoreactivity was observed in secretory granules
of both type 1 and type 2 cells. Our findings may indicate that type 1 cells are the main source of hypocalcin, but that type
2 cells also produce it, suggesting that the presence of two cell types reflects different physiological conditions of a single
cell type, rather than functionally different cell types.
In addition, we summarize our recent data on the localization of other calcium regulatory, or putative calcium regulatory,
hormones in fish: parathyroid hormone, calcitonin and calcitonin gene-related peptide. |
| |
Keywords: | parathyroid hormone (PTH) calcitonin calcitonin gene-related peptide (CGRP) hypocalcin radioimmunoassay immunocytochemistry |
本文献已被 SpringerLink 等数据库收录! |
|