| 农药氰戊菊酯和杀虫双诱导家蚕血液蛋白的变化 |
| |
| 引用本文： | 吴立娜,赵巧玲,裘智勇,覃光星,夏定国,沈中元,沈兴家,郭锡杰.农药氰戊菊酯和杀虫双诱导家蚕血液蛋白的变化[J].蚕业科学,2010,36(2):262-267. |
| |
| 作者姓名： | 吴立娜 赵巧玲 裘智勇 覃光星 夏定国 沈中元 沈兴家 郭锡杰 |
| |
| 作者单位： | 江苏科技大学,江苏镇江,212003;江苏科技大学,江苏镇江,212003;中国农业科学院蚕业研究所,农业部蚕桑遗传改良重点开放实验室,江苏镇江,212018 |
| |
| 基金项目： | 国家重点基础研究发展计划"973"项目 |
| |
| 摘 要： | 为了解析家蚕对常用农药氰戊菊酯和杀虫双的解毒机制,分别给家蚕5龄幼虫添食2种农药,观察家蚕对农药的中毒症状,采用双向电泳(2-DE)技术对添食农药的家蚕幼虫的血液蛋白进行分离,利用电喷雾电离(ESI)质谱对具有2次以上重复的显著差异蛋白点进行鉴定,并用生物信息学方法进行分析。家蚕5龄幼虫分别添食1/4LD50剂量浓度的2种农药后,均在3～12h内出现中毒症状,且血液蛋白的表达也发生了变化:在氰戊菊酯诱导下,幼虫血液中增加了纤维样蛋白、果糖-1,6-二磷酸醛缩酶、半胱氨酸蛋白酶抑制剂;在杀虫双诱导下,幼虫血液中增加了丝氨酸蛋白酶抑制剂-13;经2种农药分别诱导处理后3h,幼虫血液中都减少了体液低分子蛋白-Ⅲ的表达。推测家蚕对氰戊菊酯和杀虫双具有一定的免疫反应。
|
| 关 键 词： | 家蚕 血液蛋白 氰戊菊酯 杀虫双 诱导 双向电泳 ESI质谱 |
Changes of Haemolymph Proteins in Silkworm (Bombyx mori) Induced by Pesticides Fenvalerate and Dimehypo |
| |
| WU Li-Na,ZHAO Qiao-Ling,QIU Zhi-Yong,QIN Guang-Xing,XIA Ding-Guo,SHEN Zhong-Yuan,SHEN Xing-Jia,GUO Xi-Jie.Changes of Haemolymph Proteins in Silkworm (Bombyx mori) Induced by Pesticides Fenvalerate and Dimehypo[J].Acta Sericologica Sinica,2010,36(2):262-267. |
| |
| Authors: | WU Li-Na ZHAO Qiao-Ling QIU Zhi-Yong QIN Guang-Xing XIA Ding-Guo SHEN Zhong-Yuan SHEN Xing-Jia GUO Xi-Jie |
| |
| Institution: | WU Li-Na1 ZHAO Qiao-Ling1,2 QIU Zhi-Yong1,2 QIN Guang-Xing1,2 XIA Ding-Guo1,2 SHEN Zhong-Yuan1,2 SHEN Xing-Jia1,2 GUO Xi-Jie1,2(1Jiangsu University of Science , Technology,Zhenjiang Jiangsu 212003,China,2The Key Laboratory of Genetic Improvement of Silkworm , Mulberry,Ministry of Agriculture,The Sericultural Research Institute,Chinese Academy of Agricultural Sciences,Zhenjiang Jiangsu 212018,China) |
| |
| Abstract: | In order to investigate the detoxification mechanisms of silkworm(Bombyx mori)against Fenvalerate and Dimehypo,two pesticides in common use,silkworm larvae of the 5th instar were administered with these two pesticides to observe their poisoning symptoms.Two dimensional electrophoresis was employed to isolate haemolymph proteins from the poisoned larvae.The protein spots with significant difference which recurred over two times were identified through electrospray ionization mass spectrometry and were subseq... |
| |
| Keywords: | Bombyx mori Haemolymph Fenvalerate Dimehypo Induction Two-dimensional electrophoresis Electrospray ionization mass spectrometry |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
